التفاصيل البيبلوغرافية
| العنوان: |
Direct activation of airway sensory C‐fibers by SARS‐CoV‐2 S1 spike protein |
| المؤلفون: |
Joyce S. Kim, Fei Ru, Sonya Meeker, Bradley J. Undem |
| المصدر: |
Physiological Reports, Vol 11, Iss 24, Pp n/a-n/a (2023) |
| بيانات النشر: |
Wiley, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Physiology |
| مصطلحات موضوعية: |
C‐fiber, cough, sensory nerve, vagus, virus infection, Physiology, QP1-981 |
| الوصف: |
Abstract Respiratory viral infection can lead to activation of sensory afferent nerves as indicated by the consequential sore throat, sneezing, coughing, and reflex secretions. In addition to causing troubling symptoms, sensory nerve activation likely accelerates viral spreading. The mechanism how viruses activate sensory nerve terminals during infection is unknown. In this study, we investigate whether coronavirus spike protein activates sensory nerves terminating in the airways. We used isolated vagally‐innervated mouse trachea‐lung preparation for two‐photon microscopy and extracellular electrophysiological recordings. Using two‐photon Ca2+ imaging, we evaluated a total number of 786 vagal bronchopulmonary nerves in six experiments. Approximately 49% of the sensory fibers were activated by S1 protein (4 μg/mL intratracheally). Extracellular nerve recording showed the S1 protein evoked action potential discharge in sensory C‐fibers; of 39 airway C‐fibers (one fiber per mouse), 17 were activated. Additionally, Fura‐2 Ca2+ imaging was performed on neurons dissociated from vagal sensory ganglia (n = 254 from 22 mice). The result showed that 63% of neurons responded to S1 protein. SARS‐CoV‐2 S1 protein can lead to direct activation of sensory C‐fiber nerve terminals in the bronchopulmonary tract. Direct activation of C‐fibers may contribute to coronavirus symptoms, and amplify viral spreading in a population. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2051-817X |
| Relation: |
https://doaj.org/toc/2051-817X |
| DOI: |
10.14814/phy2.15900 |
| URL الوصول: |
https://doaj.org/article/e8faeabc8ff140a180c8c734dfc64fea |
| رقم الانضمام: |
edsdoj.8faeabc8ff140a180c8c734dfc64fea |
| قاعدة البيانات: |
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