Academic Journal
MicroRNA-30b is involved in the pathological process of diabetes mellitus induced by pancreatic cancer by regulating plasminogen activator inhibitor-1
| العنوان: | MicroRNA-30b is involved in the pathological process of diabetes mellitus induced by pancreatic cancer by regulating plasminogen activator inhibitor-1 |
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| المؤلفون: | Wei Yan, He Zheng, Jun Dong, Chao Liu, Zhongfu Zuo, Xuezheng Liu |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 1741-1749 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | microrna-30b, diabetes mellitus, pancreatic cancer, plasminogen activator inhibitor-1, Biotechnology, TP248.13-248.65 |
| الوصف: | This study investigated the expression of plasminogen activator inhibitor-1 (PAI-1) and microRNA (miRNA or miR)-30b in tumor tissues, blood and urine from pancreatic cancer (PC) patients with diabetes mellitus. The effect of culture supernatant of PC cells with up-regulated expression of miR-30b on β cells was also investigated. Thirty-one PC patients with diabetes mellitus and 22 PC patients without diabetes mellitus were included. PC tissues and tumor-adjacent tissues, peripheral blood and urine were collected from all PC patients. Quantitative real-time polymerase chain reaction was used to determine the mRNA and miR-30b expression; Western blotting, to determine the expression of PAI-1 protein in tissues; enzyme-linked immunosorbent assay, to determine the PAI-1 content in serum or urine and dual luciferase reporter assay, to identify direct interaction between miR-30b and PAI-1 mRNA. The proliferation of INS-1 cells was determined by MTT assay. The results showed that PAI-1 mRNA and protein expression was elevated in PC patients with diabetes in contrast to that in PC patients without diabetes. By contrast, miR-30b expression was decreased in PC patients with diabetes. miR-30b could bind with the 3′-UTR seed region of PAI-1 mRNA to regulate its expression. Overexpression of miR-30b inhibited the expression of PAI-1, altered the microenvironment surrounding PC cells and promoted the proliferation of islet β-cells. These results suggest that the expression of PAI-1 in PC patients with diabetes is up-regulated, being correlated with the reduced expression of miR-30b. miR-30b is involved in the pathological process of diabetes mellitus induced by PC by regulating PAI-1. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2019.1698977 |
| URL الوصول: | https://doaj.org/article/8f7b2abdc9dc409685a6949d9df975ee |
| رقم الانضمام: | edsdoj.8f7b2abdc9dc409685a6949d9df975ee |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2019.1698977 |