Academic Journal
Prevention and cure of murine C. difficile infection by a Lachnospiraceae strain
| العنوان: | Prevention and cure of murine C. difficile infection by a Lachnospiraceae strain |
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| المؤلفون: | Juan Noriega Tejada, William A. Walters, Yanling Wang, Melissa Kordahi, Benoit Chassaing, Joseph Pickard, Gabriel Nunez, Ruth Ley, Andrew T. Gewirtz |
| المصدر: | Gut Microbes, Vol 16, Iss 1 (2024) |
| بيانات النشر: | Taylor & Francis Group, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Diseases of the digestive system. Gastroenterology |
| مصطلحات موضوعية: | Clostridioides difficile, intestinal microbiota, fecal microbial transplant, bacteriocin, Diseases of the digestive system. Gastroenterology, RC799-869 |
| الوصف: | We sought to better understand how intestinal microbiota confer protection against Clostridioides difficile (C. difficile) infection (CDI). We utilized gnotobiotic altered Schaedler flora (ASF) mice, which lack the abnormalities of germfree (GF) mice as well as the complexity and heterogeneity of antibiotic-treated mice. Like GF mice, ASF mice were highly prone to rapid lethal CDI, without antibiotics, while very low infectious doses resulted in chronic CDI. Administering such chronic CDI mice an undefined preparation of Clostridia lowered C. difficile levels by several logs. Importantly, such resolution of CDI was associated with colonization of Lachnospiraceae. Fractionation of the Clostridia population to enrich for Lachnospiraceae led to the appreciation that its CDI-impeding property strongly associated with a specific Lachnospiraceae strain, namely uncultured bacteria and archaea (UBA) 3401. UBA3401 was recalcitrant to being propagated as a pure culture but could be maintained in ASF mice, wherein it comprised up to about 50% of the intestinal microbiota, which was sufficient to generate a high-quality genomic sequence of this bacterium. Sequence analysis and ex vivo study of UBA3401 indicated that it had the ability to secrete substance(s) that directly impeded C. difficile growth. Moreover, in vivo administration of UBA3401/ASF feces provided strong protection to C. difficile challenge. Thus, UBA3401 may contribute to and/or provide a means to study microbiota-mediated CDI resistance. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 19490976 1949-0984 1949-0976 |
| Relation: | https://doaj.org/toc/1949-0976; https://doaj.org/toc/1949-0984 |
| DOI: | 10.1080/19490976.2024.2392872 |
| URL الوصول: | https://doaj.org/article/8ee09a0a1d08425bae159a8178635589 |
| رقم الانضمام: | edsdoj.8ee09a0a1d08425bae159a8178635589 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19490976 19490984 |
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| DOI: | 10.1080/19490976.2024.2392872 |