Academic Journal
Down-regulation of miR-210-3p encourages chemotherapy resistance of renal cell carcinoma via modulating ABCC1
| العنوان: | Down-regulation of miR-210-3p encourages chemotherapy resistance of renal cell carcinoma via modulating ABCC1 |
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| المؤلفون: | Songchao Li, Jinjian Yang, Jun Wang, Wansheng Gao, Yafei Ding, Yinghui Ding, Zhankui Jia |
| المصدر: | Cell & Bioscience, Vol 8, Iss 1, Pp 1-10 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biotechnology LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Renal cell carcinoma, Multi-drug resistance, MiR-210-3p, ABCC1, MDR-1, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | Abstract Background ATP-binding cassette transporter super-family including ABCC1 and MDR-1 were involved in multi-drug resistance (MDR) of renal cell carcinoma (RCC) patients. Several miRNAs were confirmed to promote the MDR and the survival of tumor cells. Methods The RCC cell lines Caki-2 with vinblastine-resistant (Caki-2/VBL) or doxorubicin-resistant (Caki-2/DOX) were constructed, respectively. The expressions of miR-210-3p, ABCC1 and MDR-1 protein were determined by qRT-PCR and Western blot assays. The viability of RCC cells was assessed by MTT assay. The regulatory relationship between miR-210-3p and ABCC1 was analyzed by Dual Luciferase assay. The effect of miR-210-3p in vivo was investigated with a tumor xenograft model in mice. Results MiR-210-3p expression was observed to significantly decrease in Caki-2/VBL and Caki-2/DOX cells. Meanwhile, ABCC1 and MDR-1 were significantly increased in Caki-2/VBL and Caki-2/DOX cells. ABCC1 was a novel target of miR-210-3p and negatively regulated by miR-210-3p. And miR-210-3p improved drug-sensitivity of RCC cells. Down-regulation of ABCC1 could reverse the effect of miR-210-3p knockdown on the drug-resistance and the level of MDR-1 in drug-sensitive RCC cells. Conclusion We confirmed that down-regulation of miR-210-3p increased ABCC1 expression, thereby enhancing the MRP-1-mediated multidrug resistance of RCC cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-3701 |
| Relation: | http://link.springer.com/article/10.1186/s13578-018-0209-3; https://doaj.org/toc/2045-3701 |
| DOI: | 10.1186/s13578-018-0209-3 |
| URL الوصول: | https://doaj.org/article/c8db7b6908fb4fea8bfcd83256b8fb99 |
| رقم الانضمام: | edsdoj.8db7b6908fb4fea8bfcd83256b8fb99 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20453701 |
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| DOI: | 10.1186/s13578-018-0209-3 |