التفاصيل البيبلوغرافية
| العنوان: |
Variability in plasma donor-derived cell-free DNA levels with CLAD more than 5-years after Lung Transplantation: Pilot data |
| المؤلفون: |
Deborah Jo Levine, Zachary P. Demko, David J. Ross |
| المصدر: |
Transplantation Reports, Vol 7, Iss 3, Pp 100106- (2022) |
| بيانات النشر: |
Elsevier, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Surgery |
| مصطلحات موضوعية: |
Cell-free DNA, CLAD, Lung transplant, Biomarker, Surgery, RD1-811 |
| الوصف: |
Background: Chronic lung allograft dysfunction (CLAD) is a highly prevalent and devastating complication in lung transplant (LT), culminating in increased allograft failure, morbidity, and mortality. Determination of the fraction of plasma donor-derived cell-free DNA (dd-cfDNA) has emerged as a valuable noninvasive monitoring tool after LT; however, the increased variance of host cfDNA caused by infection and other inflammation can complicate the approach. Methods: In a retrospective pilot study, we analyzed both the fraction of dd-cfDNA (%dd-cfDNA) and absolute quantity of dd-cfDNA (cp/mL) in recipients with CLAD ≥ 5-year post-LT with co-morbid conditions (gastro-esophageal reflux, antibody-mediated rejection, or chronic infection) designated as complicated (C-CLAD) and uncomplicated (U-CLAD) cohorts. Results: Median time post-LT was 2,149 days (1,899-2,920). The median %dd-cfDNA for the C-CLAD (N=5) cohort was 1.79% (IQR: 1.04-2.29) and significantly elevated compared to the U-CLAD cohort (N=7, 0.49%; 0.28-0.88) (p=0.018). Absolute dd-cfDNA was also significantly higher in C-CLAD (43.2 cp/mL; 27.9-89.3) than the in U-CLAD cohort (19.6 cp/mL; 8.1-27.9) (p=0.048). Conclusions: We report a heretofore undescribed dichotomy of dd-cfDNA levels with CLAD ≥ 5-years, related specifically to elevation in allograft quantity as opposed to alteration in host plasma cfDNA. Further, dd-cfDNA analysis in association with co-morbid conditions in C-CLAD may offer insights for potential treatment and alleviation of molecular injury. Measurement of longitudinal absolute quantity dd-cfDNA may provide additional value for future clinical study design of pathobiology and CLAD treatment algorithms. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2451-9596 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2451959622000142; https://doaj.org/toc/2451-9596 |
| DOI: |
10.1016/j.tpr.2022.100106 |
| URL الوصول: |
https://doaj.org/article/89afa3fb30494295959ce02a31081ec2 |
| رقم الانضمام: |
edsdoj.89afa3fb30494295959ce02a31081ec2 |
| قاعدة البيانات: |
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