Academic Journal
Comparative Semen Microbiota Composition of a Stallion in a Taylorella equigenitalis Carrier and Non-Carrier State
| العنوان: | Comparative Semen Microbiota Composition of a Stallion in a Taylorella equigenitalis Carrier and Non-Carrier State |
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| المؤلفون: | Carlota Quiñones-Pérez, Amparo Martínez, Francisco Crespo, José Luis Vega-Pla |
| المصدر: | Animals, Vol 10, Iss 5, p 868 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Veterinary medicine LCC:Zoology |
| مصطلحات موضوعية: | Taylorella equigenitalis, carrier, microbiome, stallion, Veterinary medicine, SF600-1100, Zoology, QL1-991 |
| الوصف: | Contagious equine metritis is receiving renewed attention due to the continuous detection of carriers in apparent agent-free farms. Interactions of Taylorella with the seminal microflora may be the plausible cause behind these spontaneous changes of the carrier state. Accordingly, the aim of this study was to compare the differences in the seminal microbiome composition of one stallion in the contagious equine metritis carrier state and non-carrier state. Samples were cryopreserved after their extraction. Cell disruption was performed by high-speed homogenization in grinding media. Bacterial families were identified via V3 amplification of the 16S rRNA gene and Ion Torrent sequencing. Only bacterial families with relative abundance above 5% were taken into consideration. The positive sample contained a strong dominance of Corynebacteriaceae (37.75%) and Peptoniphilaceae (28.56%). In the negative sample, the Porphyromonadaceae (20.51%), Bacteroidaceae (19.25%) and Peptoniphilaceae (18.57%) families prevailed. In conclusion, the microbiome seminal composition varies when an individual carries Taylorella from when it is free of it. The wider differences were found in the Corynebacteriaceae, Porphyromonadaceae and Bacteroidaceae families. Due to the limitations of a single-case analysis, further studies are needed for a better understanding of the stallion seminal microflora interactions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 10050868 2076-2615 |
| Relation: | https://www.mdpi.com/2076-2615/10/5/868; https://doaj.org/toc/2076-2615 |
| DOI: | 10.3390/ani10050868 |
| URL الوصول: | https://doaj.org/article/89ac63056df84097abd418c0f420bce0 |
| رقم الانضمام: | edsdoj.89ac63056df84097abd418c0f420bce0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10050868 20762615 |
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| DOI: | 10.3390/ani10050868 |