التفاصيل البيبلوغرافية
| العنوان: |
Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe‐host‐communication in vivo |
| المؤلفون: |
Miriam Bittel, Patrick Reichert, Ilann Sarfati, Anja Dressel, Stefanie Leikam, Stefan Uderhardt, Iris Stolzer, Tuan Anh Phu, Martin Ng, Ngan K. Vu, Stefan Tenzer, Ute Distler, Stefan Wirtz, Veit Rothhammer, Markus F. Neurath, Robert L. Raffai, Claudia Günther, Stefan Momma |
| المصدر: |
Journal of Extracellular Vesicles, Vol 10, Iss 12, Pp n/a-n/a (2021) |
| بيانات النشر: |
Wiley, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Cytology |
| مصطلحات موضوعية: |
Cre‐Loxp, extracellular vesicles, inflammation, intestinal stem cells, microbiome, outer membrane vesicles, Cytology, QH573-671 |
| الوصف: |
Abstract The intestinal microbiota influences mammalian host physiology in health and disease locally in the gut but also in organs devoid of direct contact with bacteria such as the liver and brain. Extracellular vesicles (EVs) or outer membrane vesicles (OMVs) released by microbes are increasingly recognized for their potential role as biological shuttle systems for inter‐kingdom communication. However, physiologically relevant evidence for the transfer of functional biomolecules from the intestinal microbiota to individual host cells by OMVs in vivo is scarce. By introducing Escherichia coli engineered to express Cre‐recombinase (E. coliCre) into mice with a Rosa26.tdTomato‐reporter background, we leveraged the Cre‐LoxP system to report the transfer of bacterial OMVs to recipient cells in vivo. Colonizing the intestine of these mice with E. coliCre, resulted in Cre‐recombinase induced fluorescent reporter gene‐expression in cells along the intestinal epithelium, including intestinal stem cells as well as mucosal immune cells such as macrophages. Furthermore, even far beyond the gut, bacterial‐derived Cre induced extended marker gene expression in a wide range of host tissues, including the heart, liver, kidney, spleen, and brain. Together, our findings provide a method and proof of principle that OMVs can serve as a biological shuttle system for the horizontal transfer of functional biomolecules between bacteria and mammalian host cells. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2001-3078 |
| Relation: |
https://doaj.org/toc/2001-3078 |
| DOI: |
10.1002/jev2.12159 |
| URL الوصول: |
https://doaj.org/article/87b15044e4634669bd9079a80e391710 |
| رقم الانضمام: |
edsdoj.87b15044e4634669bd9079a80e391710 |
| قاعدة البيانات: |
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