Academic Journal
Integration of transcriptomics and metabolomics to identify key coumarin biosynthetic genes in Bupleurum chinense
| العنوان: | Integration of transcriptomics and metabolomics to identify key coumarin biosynthetic genes in Bupleurum chinense |
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| المؤلفون: | Bin Yang, Shi-Hang Liu, Yilian He, Yuchan Li, Liang Feng, Meng Zhang, Jun Zhao, Yiguan Zhang, Xia Yu, Hua Chen, Dabin Hou, Junning Zhao, Ma Yu |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1858-1868 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | bupleurum chinense dc, coumarins, key genes, transcriptome, metabolome, Biotechnology, TP248.13-248.65 |
| الوصف: | Bupleurum chinense DC. is a perennial herb plant belonging to the Umbelliferae family, genus Bupleurum, that is used as a traditional Chinese medicine. Coumarins are characteristic secondary metabolites of the Apiaceous family, which possess anti-inflammatory, anti-cancer and antiviral activities, among others. Few studies on the coumarin biosynthesis pathways in B. Chinese have been reported. In this study, we identified 89 differentially expressed genes belonging to 7 gene families involved in the synthesis of coumarins in B. chinense, using transcriptome and metabolomic data obtained by high-throughput RNA-sequencing data analysis, and liquid chromatography tandem mass spectrometry. Five coumarin compounds: esculetin (2), fraxetin (4), psoralen (5), parmesan (6) and angelicin (7) were identified through metabolomic analysis. A total of 39 unigenes belonging to five gene families: shikimate O-hydroxycinnamoyltransferase (HCT), caffeic acid ο-methyltransferase (COMT), 4-coumarate: coenzyme A ligase (4CL), feruloyl-CoA 6’-hydroxylase (F6’H) and psoralen synthase (PS), were identified by correlating coumarin content with transcriptional changes. According to correlation network analysis, PS genes (Bc09210, Bc12711), COMT genes (Bc14830, Bc17539, Bc28792, Bc32856) and HCT genes (Bc32814, Bc10245) may be important factors affecting coumarin biosynthesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2021.2023327 |
| URL الوصول: | https://doaj.org/article/8799219dea8a462fa8416e04037dd698 |
| رقم الانضمام: | edsdoj.8799219dea8a462fa8416e04037dd698 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2021.2023327 |