التفاصيل البيبلوغرافية
| العنوان: |
The C-Terminal Domain of Nefmut Is Dispensable for the CD8+ T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles |
| المؤلفون: |
Chiara Chiozzini, Francesco Manfredi, Flavia Ferrantelli, Patrizia Leone, Andrea Giovannelli, Eleonora Olivetta, Maurizio Federico |
| المصدر: |
Vaccines, Vol 9, Iss 4, p 373 (2021) |
| بيانات النشر: |
MDPI AG, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Medicine |
| مصطلحات موضوعية: |
extracellular vesicles, HIV-1 Nef, DNA immunization, CD8+ T cell immunity, HPV vaccines, SARS-CoV-2 vaccines, Medicine |
| الوصف: |
Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein Nefmut fused at its C-terminus to viral and tumor antigens elicit a potent, effective, and anti-tolerogenic CD8+ T cell immunity against the heterologous antigen. The immune response is induced through the production of EVs incorporating Nefmut-derivatives released by muscle cells. In the perspective of a possible translation into the clinic of the Nefmut-based vaccine platform, we aimed at increasing its safety profile by identifying the minimal part of Nefmut retaining the EV-anchoring protein property. We found that a C-terminal deletion of 29-amino acids did not affect the ability of Nefmut to associate with EVs. The EV-anchoring function was also preserved when antigens from both HPV16 (i.e., E6 and E7) and SARS-CoV-2 (i.e., S1 and S2) were fused to its C-terminus. Most important, the Nefmut C-terminal deletion did not affect levels, quality, and diffusion at distal sites of the antigen-specific CD8+ T immunity. We concluded that the C-terminal Nefmut truncation does not influence stability, EV-anchoring, and CD8+ T cell immunogenicity of the fused antigen. Hence, the C-terminal deleted Nefmut may represent a safer alternative to the full-length isoform for vaccines in humans. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2076-393X |
| Relation: |
https://www.mdpi.com/2076-393X/9/4/373; https://doaj.org/toc/2076-393X |
| DOI: |
10.3390/vaccines9040373 |
| URL الوصول: |
https://doaj.org/article/863f59beec024b64840311d45176b793 |
| رقم الانضمام: |
edsdoj.863f59beec024b64840311d45176b793 |
| قاعدة البيانات: |
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