التفاصيل البيبلوغرافية
| العنوان: |
Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-κB pathway |
| المؤلفون: |
Qing Yang, Binhui Xie, Hui Tang, Wei Meng, Changchang Jia, Xiaomei Zhang, Yi Zhang, Jianwen Zhang, Heping Li, Binsheng Fu |
| المصدر: |
Journal of Experimental & Clinical Cancer Research, Vol 38, Iss 1, Pp 1-12 (2019) |
| بيانات النشر: |
BMC, 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: |
MCM3, HCC, Radiotherapy resistance, NF-κB pathway, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: |
Abstract Background Hepatocellular carcinoma (HCC) is the most common tumors in the worldwide, it develops resistance to radiotherapy during treatment, understanding the regulatory mechanisms of radioresistance generation is the urgent need for HCC therapy. Methods qRT-PCR, western blot and immunohistochemistry were used to examine MCM3 expression. MTT assay, colony formation assay, terminal deoxynucleotidyl transferase nick end labeling assay and In vivo xenograft assay were used to determine the effect of MCM3 on radioresistance. Gene set enrichment analysis, luciferase reporter assay, western blot and qRT-PCR were used to examine the effect of MCM3 on NF-κB pathway. Results We found DNA replication initiation protein Minichromosome Maintenance 3 (MCM3) was upregulated in HCC tissues and cells, patients with high MCM3 expression had poor outcome, it was an independent prognostic factor for HCC. Cells with high MCM3 expression or MCM3 overexpression increased the radioresistance determined by MTT assay, colony formation assay, TUNEL assay and orthotopic transplantation mouse model, while cells with low MCM3 expression or MCM3 knockdown reduced the radioresistance. Mechanism analysis showed MCM3 activated NF-κB pathway, characterized by increasing the nuclear translocation of p65, the expression of the downstream genes NF-κB pathway and the phosphorylation of IKK-β and IκBα. Inhibition of NF-κB in MCM3 overexpressing cells using small molecular inhibitor reduced the radioresistance, suggesting MCM3 increased radioresistance through activating NF-κB pathway. Moreover, we found MCM3 expression positively correlated with NF-κB pathway in clinic. Conclusions Our findings revealed that MCM3 promoted radioresistance through activating NF-κB pathway, strengthening the role of MCM subunits in the tumor progression and providing a new target for HCC therapy. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1756-9966 |
| Relation: |
http://link.springer.com/article/10.1186/s13046-019-1241-9; https://doaj.org/toc/1756-9966 |
| DOI: |
10.1186/s13046-019-1241-9 |
| URL الوصول: |
https://doaj.org/article/83bb8b9d0c8d4ac995b5d1a982047783 |
| رقم الانضمام: |
edsdoj.83bb8b9d0c8d4ac995b5d1a982047783 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder