Academic Journal
Storage of Mycobacterium tuberculosis culture isolates in MicrobankTM beads at a South African laboratory
| العنوان: | Storage of Mycobacterium tuberculosis culture isolates in MicrobankTM beads at a South African laboratory |
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| المؤلفون: | Anura David, Lesley E. Scott, Pedro Da Silva, Elizabeth Mayne, Wendy S. Stevens |
| المصدر: | African Journal of Laboratory Medicine, Vol 12, Iss 1, Pp e1-e6 (2023) |
| بيانات النشر: | AOSIS, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Public aspects of medicine LCC:Medicine (General) |
| مصطلحات موضوعية: | mycobacterium tuberculosis, culture isolates, storage, liquid culture, microbank beads, Public aspects of medicine, RA1-1270, Medicine (General), R5-920 |
| الوصف: | Background: Mycobacterium tuberculosis complex (MTBC) isolates are typically stored at −70 °C in cryovials containing 1 mL aliquots of a liquid medium, with or without 50% glycerol. Multiple uses of the culture stock may decrease the strain viability while increasing the risk of culture contamination. Small culture aliquots may be more practical; however, storage capacity remains challenging. MicrobankTM beads (25 beads/vial) for the long-term storage of fungal cultures is well documented, but their use for storing MTBC isolates is uninvestigated. Objective: The study aimed to determine the feasibility of using MicrobankTM beads for long-term storage of MTBC isolates at a laboratory in South Africa. Methods: In February 2020, 20 isolates in liquid culture were stored in MicrobankTM beads, following an in-house developed protocol, at −70 °C. At defined time points (16 months [15 June 2021] and 21 months [18 November 2021]), two beads were retrieved from each storage vial and assessed for viability and level of contamination. Results: Stored liquid isolates demonstrated MTBC growth within an average time-to-detection of 18 days following retrieval, even at 21 months post storage. Contaminating organisms were detected in 2 of 80 (2.5%) culture isolates. Conclusion: MicrobankTM beads will allow for the reculture of up to 25 culture isolates using a reduced culture volume compared to current storage methods. MicrobankTM beads represent a storage solution for the medium-term storage of MTBC isolates. What this study adds: This study evaluated the use of MicrobankTM beads as an alternate method for storing MTBC culture isolates at −70 °C and provided a suitable option for medium-term storage of MTBC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2225-2002 2225-2010 |
| Relation: | https://ajlmonline.org/index.php/ajlm/article/view/2172; https://doaj.org/toc/2225-2002; https://doaj.org/toc/2225-2010 |
| DOI: | 10.4102/ajlm.v12i1.2172 |
| URL الوصول: | https://doaj.org/article/839dfdf6792b410b993c44e51ee49970 |
| رقم الانضمام: | edsdoj.839dfdf6792b410b993c44e51ee49970 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22252002 22252010 |
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| DOI: | 10.4102/ajlm.v12i1.2172 |