Academic Journal
VITAMIN D3 AFFECTS GLUCOCORTICOID-SENSITIVE RECEPTORS AND BRAIN-DERIVED NEUROTROPHIC FACTOR IN PREDNISOLONE-INDUCED NEUROTOXICITY
| العنوان: | VITAMIN D3 AFFECTS GLUCOCORTICOID-SENSITIVE RECEPTORS AND BRAIN-DERIVED NEUROTROPHIC FACTOR IN PREDNISOLONE-INDUCED NEUROTOXICITY |
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| المؤلفون: | D.I. YEVSTIFEIEV, O.O. LISAKOVSKA, A.V. KHOMENKO, I.O. SHYMANSKYI |
| المصدر: | Biotechnologia Acta, Vol 17, Iss 2, Pp 87-89 (2024) |
| بيانات النشر: | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | glucocorticoid-induced neurotoxicity, prednisolone, vitamin d3, brain-derived neurotrophic factor, steroid receptors, astrocytes., Biotechnology, TP248.13-248.65 |
| الوصف: | Aim. The study aimed to explore the effects of vitamin D3 (D3) on markers of brain tissue metabolism in prednisolone-induced neurotoxicity in rats. Methods. Female Wistar rats received prednisolone (5 mg/kg of b.w.) with or without D3 (1000 IU/kg of b.w.) for 30 days. Serum 25-hydroxyvitamin D3 (25D3) and brain tissue BDNF levels were measured by ELISA. We used western blotting to determine levels of glucocorticoid-sensitive receptors: GRα/β (glucocorticoid receptor) and MR (mineralocorticoid receptor). The number of astrocytes in histological sections of the cerebral cortex and hippocampus CA1-CA3 regions was assessed by immunofluorescent labeling of the macroglial marker protein GFAP (glial fibrillary acidic protein). Data were statistically analyzed using one-way ANOVA followed by Tukey’s test. The significant level was set at P < 0.05. Results. Long-term administration of prednisolone decreased serum and brain tissue 25D3 levels and increased the GR/MR ratio, suggesting a potential neurotoxic effect. It also increased brain tissue BDNF levels and astrocyte numbers in histological sections of rats` brains. D3 supplementation completely or partially reversed the alterations, elucidating its neuroprotective effect. Conclusions. The study suggests that D3 deficiency may contribute to neuropathological changes induced by long-term exposure to prednisolone. Based on the identified positive effects of D3 on the CNS, its practical usefulness in the complex treatment of neurological and cognitive disorders associated with GC-based therapeutics can be envisaged. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Ukrainian |
| تدمد: | 2410-7751 2410-776X |
| Relation: | https://biotechnology.kiev.ua/images/BTA/2024/2_2024/Yevstifeiev_2_2024.pdf; https://doaj.org/toc/2410-7751; https://doaj.org/toc/2410-776X |
| DOI: | 10.15407/biotech17.02.087 |
| URL الوصول: | https://doaj.org/article/839d1bb83f974601a957fc35092c9e12 |
| رقم الانضمام: | edsdoj.839d1bb83f974601a957fc35092c9e12 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 24107751 2410776X |
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| DOI: | 10.15407/biotech17.02.087 |