Academic Journal
Sexually dimorphic DNA methylation and gene expression patterns in human first trimester placenta
| العنوان: | Sexually dimorphic DNA methylation and gene expression patterns in human first trimester placenta |
|---|---|
| المؤلفون: | Tania L. Gonzalez, Bryn E. Willson, Erica T. Wang, Kent D. Taylor, Allynson Novoa, Akhila Swarna, Juanita C. Ortiz, Gianna J. Zeno, Caroline A. Jefferies, Kate Lawrenson, Jerome I. Rotter, Yii-Der Ida Chen, John Williams, Jinrui Cui, Mark O. Goodarzi, Margareta D. Pisarska |
| المصدر: | Biology of Sex Differences, Vol 15, Iss 1, Pp 1-17 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Physiology |
| مصطلحات موضوعية: | Placenta, Early pregnancy, Healthy pregnancy, Fetal sex, DNA methylation, Total RNA-seq, Medicine, Physiology, QP1-981 |
| الوصف: | Abstract Background Fetal sex and placental development impact pregnancy outcomes and fetal–maternal health, but the critical timepoint of placenta establishment in first trimester is understudied in human pregnancies. Methods Pregnant subjects were recruited in late first trimester (weeks 10–14) at time of chorionic villus sampling, a prenatal diagnostic test. Leftover placenta tissue was collected and stored until birth outcomes were known, then DNA and RNA were isolated from singleton, normal karyotype pregnancies resulting in live births. DNA methylation was measured with the Illumina Infinium MethylationEPIC BeadChip array (n = 56). Differential methylation analysis compared 25 females versus 31 males using a generalized linear model on 743,461 autosomal probes. Gene expression sex differences were analyzed with RNA-sequencing (n = 74). An integrated analysis was performed using linear regression to correlate gene expression and DNA methylation in 51 overlapping placentas. Results Methylation analysis identified 151 differentially methylated probes (DMPs) significant at false discovery rate |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2042-6410 |
| Relation: | https://doaj.org/toc/2042-6410 |
| DOI: | 10.1186/s13293-024-00629-9 |
| URL الوصول: | https://doaj.org/article/a81cf7101f17431ea7b627c97f152ea6 |
| رقم الانضمام: | edsdoj.81cf7101f17431ea7b627c97f152ea6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20426410 |
|---|---|
| DOI: | 10.1186/s13293-024-00629-9 |