Academic Journal
A New Approach Using the SYBR Green-Based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage
| العنوان: | A New Approach Using the SYBR Green-Based Real-Time PCR Method for Detection of Soft Rot Pectobacterium odoriferum Associated with Kimchi Cabbage |
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| المؤلفون: | Yong Ju Jin, Dawon Jo, Soon-Wo Kwon, Samnyu Jee, Jeong-Seon Kim, Jegadeesh Raman, Soo-Jin Kim |
| المصدر: | The Plant Pathology Journal, Vol 38, Iss 6, Pp 656-664 (2022) |
| بيانات النشر: | Hanrimwon Publishing Company, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Plant culture |
| مصطلحات موضوعية: | pectobacterium odoriferum, species-specific genes, sybr green real-time qpcr, Plant culture, SB1-1110 |
| الوصف: | Pectobacterium odoriferum is the primary causative agent in Kimchi cabbage soft-rot diseases. The pathogenic bacteria Pectobacterium genera are responsible for significant yield losses in crops. However, P. odoriferum shares a vast range of hosts with P. carotovorum, P. versatile, and P. brasiliense, and has similar biochemical, phenotypic, and genetic characteristics to these species. Therefore, it is essential to develop a P. odoriferum-specific diagnostic method for soft-rot disease because of the complicated diagnostic process and management as described above. Therefore, in this study, to select P. odoriferum-specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. Thereafter, the specificity of the selected gene was tested through blast analysis. We identified novel species-specific genes to detect and quantify targeted P. odoriferum and designed specific primer sets targeting HAD family hydrolases. It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. Furthermore, the population density of P. odoriferum can be estimated without genomic DNA extraction through SYBR Green-based real-time quantitative PCR using a primer set in plants. As a result, the newly developed diagnostic method enables rapid and accurate diagnosis and continuous monitoring of soft-rot disease in Kimchi cabbage without additional procedures from the plant tissue. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1598-2254 2093-9280 |
| Relation: | http://www.ppjonline.org/upload/pdf/PPJ-OA-09-2022-0138.pdf; https://doaj.org/toc/1598-2254; https://doaj.org/toc/2093-9280 |
| DOI: | 10.5423/PPJ.OA.09.2022.0138 |
| URL الوصول: | https://doaj.org/article/7fa1595c98284b30b5481ff8b0be1132 |
| رقم الانضمام: | edsdoj.7fa1595c98284b30b5481ff8b0be1132 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15982254 20939280 |
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| DOI: | 10.5423/PPJ.OA.09.2022.0138 |