التفاصيل البيبلوغرافية
| العنوان: |
A Non-Dicer RNase III and Four Other Novel Factors Required for RNAi-Mediated Transposon Suppression in the Human Pathogenic Yeast Cryptococcus neoformans |
| المؤلفون: |
Jordan E. Burke, Adam D. Longhurst, Prashanthi Natarajan, Beiduo Rao, John Liu, Jade Sales-Lee, Yasaman Mortensen, James J. Moresco, Jolene K. Diedrich, John R. Yates, Hiten D. Madhani |
| المصدر: |
G3: Genes, Genomes, Genetics, Vol 9, Iss 7, Pp 2235-2244 (2019) |
| بيانات النشر: |
Oxford University Press, 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Genetics |
| مصطلحات موضوعية: |
RNAi, transposon, RNA surveillance, Genetics, QH426-470 |
| الوصف: |
The human pathogenic yeast Cryptococcus neoformans silences transposable elements using endo-siRNAs and an Argonaute, Ago1. Endo-siRNAs production requires the RNA-dependent RNA polymerase, Rdp1, and two partially redundant Dicer enzymes, Dcr1 and Dcr2, but is independent of histone H3 lysine 9 methylation. We describe here an insertional mutagenesis screen for factors required to suppress the mobilization of the C. neoformans HARBINGER family DNA transposon HAR1. Validation experiments uncovered five novel genes (RDE1-5) required for HAR1 suppression and global production of suppressive endo-siRNAs. The RDE genes do not impact transcript levels, suggesting the endo-siRNAs do not act by impacting target transcript synthesis or turnover. RDE3 encodes a non-Dicer RNase III related to S. cerevisiae Rnt1, RDE4 encodes a predicted terminal nucleotidyltransferase, while RDE5 has no strongly predicted encoded domains. Affinity purification-mass spectrometry studies suggest that Rde3 and Rde5 are physically associated. RDE1 encodes a G-patch protein homologous to the S. cerevisiae Sqs1/Pfa1, a nucleolar protein that directly activates the essential helicase Prp43 during rRNA biogenesis. Rde1 copurifies Rde2, another novel protein obtained in the screen, as well as Ago1, a homolog of Prp43, and numerous predicted nucleolar proteins. We also describe the isolation of conditional alleles of PRP43, which are defective in RNAi. This work reveals unanticipated requirements for a non-Dicer RNase III and presumptive nucleolar factors for endo-siRNA biogenesis and transposon mobilization suppression in C. neoformans. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2160-1836 |
| Relation: |
https://doaj.org/toc/2160-1836 |
| DOI: |
10.1534/g3.119.400330 |
| URL الوصول: |
https://doaj.org/article/7f7f371b930043a1aae902382619436c |
| رقم الانضمام: |
edsdoj.7f7f371b930043a1aae902382619436c |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder