التفاصيل البيبلوغرافية
| العنوان: |
SARS-CoV-2 rapidly evolves lineage-specific phenotypic differences when passaged repeatedly in immune-naïve mice |
| المؤلفون: |
Julian Daniel Sunday Willett, Annie Gravel, Isabelle Dubuc, Leslie Gudimard, Ana Claudia dos Santos Pereira Andrade, Émile Lacasse, Paul Fortin, Ju-Ling Liu, Jose Avila Cervantes, Jose Hector Galvez, Haig Hugo Vrej Djambazian, Melissa Zwaig, Anne-Marie Roy, Sally Lee, Shu-Huang Chen, Jiannis Ragoussis, Louis Flamand |
| المصدر: |
Communications Biology, Vol 7, Iss 1, Pp 1-13 (2024) |
| بيانات النشر: |
Nature Portfolio, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Biology (General), QH301-705.5 |
| الوصف: |
Abstract The persistence of SARS-CoV-2 despite the development of vaccines and a degree of herd immunity is partly due to viral evolution reducing vaccine and treatment efficacy. Serial infections of wild-type (WT) SARS-CoV-2 in Balb/c mice yield mouse-adapted strains with greater infectivity and mortality. We investigate if passaging unmodified B.1.351 (Beta) and B.1.617.2 (Delta) 20 times in K18-ACE2 mice, expressing the human ACE2 receptor, in a BSL-3 laboratory without selective pressures, drives human health-relevant evolution and if evolution is lineage-dependent. Late-passage virus causes more severe disease, at organism and lung tissue scales, with late-passage Delta demonstrating antibody resistance and interferon suppression. This resistance co-occurs with a de novo spike S371F mutation, linked with both traits. S371F, an Omicron-characteristic mutation, is co-inherited at times with spike E1182G per Nanopore sequencing, existing in different within-sample viral variants at others. Both S371F and E1182G are linked to mammalian GOLGA7 and ZDHHC5 interactions, which mediate viral-cell entry and antiviral response. This study demonstrates SARS-CoV-2’s tendency to evolve with phenotypic consequences, its evolution varying by lineage, and suggests non-dominant quasi-species contribution. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2399-3642 |
| Relation: |
https://doaj.org/toc/2399-3642 |
| DOI: |
10.1038/s42003-024-05878-3 |
| URL الوصول: |
https://doaj.org/article/7e05d290e45d4f32a383ce6ae1b567f7 |
| رقم الانضمام: |
edsdoj.7e05d290e45d4f32a383ce6ae1b567f7 |
| قاعدة البيانات: |
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