التفاصيل البيبلوغرافية
| العنوان: |
Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells |
| المؤلفون: |
Tsvetelina E. Germanova, Emanuele Roscioli, Jonathan U. Harrison, Andrew D. McAinsh, Nigel J. Burroughs |
| المصدر: |
STAR Protocols, Vol 2, Iss 4, Pp 100774- (2021) |
| بيانات النشر: |
Elsevier, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Science (General) |
| مصطلحات موضوعية: |
Cell Biology, Cell culture, Microscopy, Science (General), Q1-390 |
| الوصف: |
Summary: This protocol measures the 3D Euclidean distance (Δ3D) between two/three fluorescently labeled kinetochore components in fixed samples using Kinetochore Delta software (KiDv1.0.1, MATLAB based). Overestimation of mean Δ3D is corrected through a Bayesian algorithm, with ΔEC distances reflecting the ensemble average positions of fluorophores within a kinetochore population. This package also enables kinetochore categorization, which can be used to sub-sample kinetochores and measure ΔEC. Together, this allows the dynamic architecture of human kinetochores to be investigated (tested in hTERT-RPE1 cells).For complete details on the use and execution of this protocol, please refer to Roscioli et al. (2020). |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166721004809; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2021.100774 |
| URL الوصول: |
https://doaj.org/article/7c410bd5af754cd2836e928d7012fddf |
| رقم الانضمام: |
edsdoj.7c410bd5af754cd2836e928d7012fddf |
| قاعدة البيانات: |
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