التفاصيل البيبلوغرافية
| العنوان: |
Detection of senescent CD8+ T-lymphocyte in newly diagnosed and relapsed/refractory multiple myeloma using CD28 and CD57 |
| المؤلفون: |
Zahraa Mahdi Sareeh, Yassmin Ali Abdul-Kareem, Ali Mohammed Jawad |
| المصدر: |
Iraqi Journal of Hematology, Vol 12, Iss 1, Pp 71-77 (2023) |
| بيانات النشر: |
Wolters Kluwer Medknow Publications, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: |
cd28, cd57, myeloma, senescent, t-lymphocytes, Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: |
BACKGROUND: Multiple myeloma is a clonal B-cell malignancy characterized by proliferation of plasma cells that secrete a complete and/or partial monoclonal immunoglobulin protein. It induces dysfunction of cytotoxic T cells that may be responsible for immune evasion and therapeutical failure of immunotherapies. T cells during senescence tend to lose co-stimulatory molecules such as CD27 and CD28 while expressing killer cell lectin-like receptor subfamily G (KLRG-1) and CD57. Therefore, enhanced knowledge about the actual status of T cells in myeloma patients is needed. Objectives: The aims of this study were to detect the senescent cytotoxic T-cell (CD8+) in patients with refractory/resistance multiple myeloma, and to compare it with newly diagnosed multiple myeloma patients, and their implications for cellular therapies. MATERIALS AND METHODS: This is a cross sectional study performed, from January to October 2021. Sixty multiple myeloma patients were sequentially chosen, thirty of them were newly diagnosed patients and another thirty were relapse/refractory who progress after receiving 2 lines of chemotherapy containing bortezomib and immunomodulators. And/or patients progressing after autologous stem cell transplantation. Seventeen apparently healthy age and gender matched adults were enrolled as a control group. Multicolor flow cytometry was utilized for the analyses of surface molecules CD 28 and CD57 on CD 8 positive T-lymphocyte using peripheral blood, subsequently the percentage of T cells senescent were estimate in CD 8+ T cells with CD28- and CD57+. Results: The mean percentage of senescent CD8 positive T lymphocyte (negative CD28 and positive CD57) were significantly higher in multiple myeloma patients than control (P |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2072-8069 |
| Relation: |
http://www.ijhonline.org/article.asp?issn=2072-8069;year=2023;volume=12;issue=1;spage=71;epage=77;aulast=Sareeh; https://doaj.org/toc/2072-8069 |
| DOI: |
10.4103/ijh.ijh_6_23 |
| URL الوصول: |
https://doaj.org/article/7bf992675088450fb676ae1beeab57e5 |
| رقم الانضمام: |
edsdoj.7bf992675088450fb676ae1beeab57e5 |
| قاعدة البيانات: |
Directory of Open Access Journals |