التفاصيل البيبلوغرافية
| العنوان: |
DbcAMP regulates adipogenesis in sheep inguinal preadipocytes |
| المؤلفون: |
Delin Kong, Jingjing Cui, Juncai Fu |
| المصدر: |
Lipids in Health and Disease, Vol 16, Iss 1, Pp 1-9 (2017) |
| بيانات النشر: |
BMC, 2017. |
| سنة النشر: |
2017 |
| المجموعة: |
LCC:Nutritional diseases. Deficiency diseases |
| مصطلحات موضوعية: |
Preadipocyte, Proliferation, Differentiation, DbcAMP, Fatty acid, mRNA, Nutritional diseases. Deficiency diseases, RC620-627 |
| الوصف: |
Abstract Background The cyclic adenosine-monophosphate (cAMP) pathway is generally recognized as one of the essential pathways for the adipose conversion of rodent preadipocytes in vitro. However, divergent effects of cAMP on adipocyte differentiation have also been reported. Since there is very little data on non-rodent preadipose cells, the aim of the present work was to analyze the effects of one classic activator (dbcAMP) of the cAMP pathway on the proliferation and differentiation of sheep preadipocytes grown. Method We retrospectively analyzed the regulation of dbcAMP on the proliferation and differentiation of sheep preadipocytes through observation on cell dynamic morphology, drawing on the growth curve, Oil Red O staining and induction of cell differentiation. Results 1) During first 5 days of treatment, at lower levels of dbcAMP (1 nmol/L to 1 × 104 nmol/L), sheep cells were not increased, but at higher levels (1 × 105 nmol/l to 1 × 106 nmol/l), they were significantly increased (P 0.05); 3) treated for 4 days, dbcAMP at the levels of 1 nmol/L, 1 × 104 nmol/L and 1 × 106 nmol/L increased C20:0 abundance (P 0.05), but expression of HSL mRNA increased at the level of 1 × 106 nmol/L dbcAMP (P |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1476-511X |
| Relation: |
http://link.springer.com/article/10.1186/s12944-017-0478-6; https://doaj.org/toc/1476-511X |
| DOI: |
10.1186/s12944-017-0478-6 |
| URL الوصول: |
https://doaj.org/article/7b4b3c8993ef427a97445877a6c65891 |
| رقم الانضمام: |
edsdoj.7b4b3c8993ef427a97445877a6c65891 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder