التفاصيل البيبلوغرافية
| العنوان: |
Novel RNA Extraction Method for Dual RNA-seq Analysis of Pathogen and Host in the Early Stages of Yersinia pestis Pulmonary Infection |
| المؤلفون: |
Ofir Israeli, Inbar Cohen-Gihon, Moshe Aftalion, David Gur, Yaron Vagima, Ayelet Zauberman, Yinon Levy, Anat Zvi, Theodor Chitlaru, Emanuelle Mamroud, Avital Tidhar |
| المصدر: |
Microorganisms, Vol 9, Iss 10, p 2166 (2021) |
| بيانات النشر: |
MDPI AG, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
dual RNA-seq, pneumonic plague, Y. pestis, mouse model, infection, Biology (General), QH301-705.5 |
| الوصف: |
Pneumonic plague, caused by Yersinia pestis, is a rapidly progressing lethal infection. The various phases of pneumonic plague are yet to be fully understood. A well-established way to address the pathology of infectious diseases in general, and pneumonic plague in particular, is to conduct concomitant transcriptomic analysis of the bacteria and the host. The analysis of dual RNA by RNA sequencing technology is challenging, due the difficulties of extracting bacterial RNA, which is overwhelmingly outnumbered by the host RNA, especially at the critical early time points post-infection (prior to 48 h). Here, we describe a novel technique that employed the infusion of an RNA preserving reagent (RNAlater) into the lungs of the animals, through the trachea, under deep anesthesia. This method enabled the isolation of stable dual mRNA from the lungs of mice infected with Y. pestis, as early as 24 h post-infection. The RNA was used for transcriptomic analysis, which provided a comprehensive gene expression profile of both the host and the pathogen. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2076-2607 |
| Relation: |
https://www.mdpi.com/2076-2607/9/10/2166; https://doaj.org/toc/2076-2607 |
| DOI: |
10.3390/microorganisms9102166 |
| URL الوصول: |
https://doaj.org/article/a797869f328d426e8f385e653ac08a35 |
| رقم الانضمام: |
edsdoj.797869f328d426e8f385e653ac08a35 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder