التفاصيل البيبلوغرافية
| العنوان: |
Single-nucleotide polymorphism screening and RNA sequencing of key messenger RNAs associated with neonatal hypoxic-ischemia brain damage |
| المؤلفون: |
Liu-Lin Xiong, Lu-Lu Xue, Mohammed Al-Hawwas, Jin Huang, Rui-Ze Niu, Ya-Xin Tan, Yang Xu, Ying-Ying Su, Jia Liu, Ting-Hua Wang |
| المصدر: |
Neural Regeneration Research, Vol 15, Iss 1, Pp 86-95 (2020) |
| بيانات النشر: |
Wolters Kluwer Medknow Publications, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Neurology. Diseases of the nervous system |
| مصطلحات موضوعية: |
CSRNP1, DUSP5, gene ontology analysis, human fetal cortical neurons, LRRC25, mRNA, neonatal hypoxic ischemic encephalopathy, pathogenesis, signaling pathway analysis, Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: |
A single-nucleotide polymorphism (SNP) is an alteration in one nucleotide in a certain position within a genome. SNPs are associated with disease susceptibility. However, the influences of SNPs on the pathogenesis of neonatal hypoxic-ischemic brain damage remain elusive. Seven-day-old rats were used to establish a hypoxic ischemic encephalopathy model. SNPs and expression profiles of mRNAs were analyzed in hypoxic ischemic encephalopathy model rats using RNA sequencing. Genes exhibiting SNPs associated with hypoxic ischemic encephalopathy were identified and studied by gene ontology and pathway analysis to identify their possible involvement in the disease mechanism. We identified 89 up-regulated genes containing SNPs that were mainly located on chromosome 1 and 2. Gene ontology analysis indicated that the up-regulated genes containing SNPs are mainly involved in angiogenesis, wound healing and glutamatergic synapse and biological processing of calcium-activated chloride channels. Signaling pathway analysis indicated that the differentially expressed genes play a role in glutamatergic synapses, long-term depression and oxytocin signaling. Moreover, intersection analysis of high throughput screening following PubMed retrieval and RNA sequencing for SNPs showed that CSRNP1, DUSP5 and LRRC25 were most relevant to hypoxic ischemic encephalopathy. Significant up-regulation of genes was confirmed by quantitative real-time polymerase chain reaction analysis of oxygen-glucose-deprived human fetal cortical neurons. Our results indicate that CSRNP1, DUSP5 and LRRC25, containing SNPs, may be involved in the pathogenesis of hypoxic ischemic encephalopathy. These findings indicate a novel direction for further hypoxic ischemic encephalopathy research. This animal study was approved on February 5, 2017 by the Animal Care and Use Committee of Kunming Medical University, Yunnan Province, China (approval No. kmmu2019038). Cerebral tissue collection from a human fetus was approved on September 30, 2015 by the Ethics Committee of Kunming Medical University, China (approval No. 2015-9). |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1673-5374 |
| Relation: |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=1;spage=86;epage=95;aulast=Xiong; https://doaj.org/toc/1673-5374 |
| DOI: |
10.4103/1673-5374.264469 |
| URL الوصول: |
https://doaj.org/article/79202ad18e184046b0c62310a90bbca2 |
| رقم الانضمام: |
edsdoj.79202ad18e184046b0c62310a90bbca2 |
| قاعدة البيانات: |
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