التفاصيل البيبلوغرافية
| العنوان: |
Association between mitochondrial DNA haplogroups J and K, serum branched-chain amino acids and lowered capability for endurance exercise |
| المؤلفون: |
Jukka M. Kiiskilä, Ilmo E. Hassinen, Johannes Kettunen, Laura Kytövuori, Ilona Mikkola, Pirjo Härkönen, Jari J. Jokelainen, Sirkka Keinänen-Kiukaanniemi, Markus Perola, Kari Majamaa |
| المصدر: |
BMC Sports Science, Medicine and Rehabilitation, Vol 14, Iss 1, Pp 1-8 (2022) |
| بيانات النشر: |
BMC, 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Sports medicine |
| مصطلحات موضوعية: |
mtDNA haplogroup, Branched-chain amino acid, Trainability, Low-responder, Military conscript, Sports medicine, RC1200-1245 |
| الوصف: |
Abstract Background Endurance exercise training promotes the catabolism of branched-chain amino acids (BCAAs) in skeletal muscles. We have previously shown that mitochondrial DNA (mtDNA) haplogroups J and K are markers of low responders in endurance training. In this paper, we hypothesize that BCAA catabolism is a surrogate marker of lower respiratory chain activity attributed to these haplogroups. We evaluated whether exercise-induced changes in amino acid concentrations differ between subjects harbouring mtDNA haplogroups J or K and those with non-JK haplogroups. Methods Finnish male conscripts (N = 633) undertook the 12-min Cooper running test at the beginning and end of their military service. The intervention during the service mainly included endurance aerobic exercise and sports-related muscle training. Concentrations of seven amino acids were analysed in the serum using a high-throughput 1H NMR metabolomics platform. Total DNA was extracted from whole blood, and restriction fragment analysis was used to determine mtDNA haplogroups J and K. Results The concentrations of the seven amino acids were higher following the intervention, with the exception of phenylalanine; interestingly, the increase in the concentrations of three BCAAs was larger in subjects with haplogroup J or K than in subjects with non-JK haplogroups (p = 0.029). MtDNA haplogroups J and K share two common nonsynonymous variants. Structural analysis based on crystallographic data on bovine complexes I and III revealed that the Leu18 variant in cytochrome b encoded by m.14798T > C may interfere with ubiquinone binding at the Qi site in complex III. Conclusions The increase in the concentrations of serum BCAAs following exercise intervention differs between subjects harbouring mtDNA haplogroup J or K and those harbouring non-JK haplogroups. Lower response in endurance training and difference in exercise-induced increase in the concentrations of serum BCAAs suggest decreased respiratory chain activity. Haplogroups J and K share m.14798T > C in MT-CYB, which may hamper the function of complex III. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2052-1847 |
| Relation: |
https://doaj.org/toc/2052-1847 |
| DOI: |
10.1186/s13102-022-00485-3 |
| URL الوصول: |
https://doaj.org/article/785a7387ecdc4629a14cf3c7d7145b2e |
| رقم الانضمام: |
edsdoj.785a7387ecdc4629a14cf3c7d7145b2e |
| قاعدة البيانات: |
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