التفاصيل البيبلوغرافية
| العنوان: |
A new approach of modified submerged patch clamp recording reveals interneuronal dynamics during epileptiform oscillations |
| المؤلفون: |
Gareth Morris, Premysl Jiruska, John Gordon Ralph Jefferys, Andrew D Powell |
| المصدر: |
Frontiers in Neuroscience, Vol 10 (2016) |
| بيانات النشر: |
Frontiers Media S.A., 2016. |
| سنة النشر: |
2016 |
| المجموعة: |
LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| مصطلحات موضوعية: |
Epilepsy, patch clamp, in vitro, LFP, high frequency activity, membrane chamber, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| الوصف: |
Traditionally, visually-guided patch clamp in brain slices using submerged recording conditions has been required to characterise the activity of individual neurons. However, due to limited oxygen availability, submerged conditions truncate fast network oscillations including epileptiform activity. Thus it is technically challenging to study the contribution of individual identified neurons to fast network activity. The membrane chamber is a submerged-style recording chamber, modified to enhance oxygen supply to the slice, which we use to demonstrate the ability to record single-cell activity during in vitro epilepsy. We elicited epileptiform activity using 9 mM potassium and simultaneously recorded from fluorescently labelled interneurons. Epileptiform discharges were more reliable than in standard submerged conditions. During these synchronous discharges interneuron firing frequency increased and action potential amplitude progressively decreased. The firing of fifteen interneurons was significantly correlated with epileptiform high frequency activity (HFA; ~100-500 Hz) cycles. We also recorded epileptiform activity in tissue prepared from chronically epileptic rats, treated with intrahippocampal tetanus neurotoxin. Four of these slices generated fast ripple activity, unique to chronic epilepsy. We showed the membrane chamber is a promising new in vitro environment facilitating patch clamp recordings in acute epilepsy models. Further, we showed that chronic epilepsy can be better modelled using ex vivo brain slices. These findings demonstrate that the membrane chamber facilitates previously challenging investigations into the neuronal correlates of epileptiform activity in vitro. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1662-453X |
| Relation: |
http://journal.frontiersin.org/Journal/10.3389/fnins.2016.00519/full; https://doaj.org/toc/1662-453X |
| DOI: |
10.3389/fnins.2016.00519 |
| URL الوصول: |
https://doaj.org/article/76bd8d3a6bcc49a7ac60b01059c09b2f |
| رقم الانضمام: |
edsdoj.76bd8d3a6bcc49a7ac60b01059c09b2f |
| قاعدة البيانات: |
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