التفاصيل البيبلوغرافية
| العنوان: |
The type 2 acyl-CoA:diacylglycerol acyltransferase family of the oleaginous microalga Lobosphaera incisa |
| المؤلفون: |
Krzysztof Zienkiewicz, Urs Benning, Heike Siegler, Ivo Feussner |
| المصدر: |
BMC Plant Biology, Vol 18, Iss 1, Pp 1-13 (2018) |
| بيانات النشر: |
BMC, 2018. |
| سنة النشر: |
2018 |
| المجموعة: |
LCC:Botany |
| مصطلحات موضوعية: |
Diacylglycerol acyltransferase, Lipids, Lipid droplet, Lobosphaera incisa, Microalgae, Botany, QK1-989 |
| الوصف: |
Abstract Background Oleaginous microalgae are promising sources of energy-rich triacylglycerols (TAGs) for direct use for food, feed and industrial applications. Lobosphaera incisa is a fresh water unicellular alga, which in response to nutrient stress accumulates a high amount of TAGs with a high proportion of arachidonic acid (ARA). The final committed step of de novo TAG biosynthesis is catalyzed by acyl-CoA:diacylglycerol acyltransferases (DGATs), which add a fatty acid (FA) to the final sn-3 position of diacylglycerol (DAG). Results Genome analysis revealed the presence of five putative DGAT isoforms in L. incisa, including one DGAT of type 1, three DGATs of type 2 and a single isoform of a type 3 DGAT. For LiDGAT1, LiDGAT2.1, LiDGAT2.2 and LiDGAT2.3 enzyme activity was confirmed by expressing them in the TAG-deficient yeast strain H1246. Feeding experiments of yeast transformants with fatty acids suggest a broad substrate specificity spectrum for LiDGAT1. A significant TAG production in response to exogenous ARA was found for LiDGAT2.2. Cellular localization of the four type 1 and type 2 DGATs expressed in yeast revealed that they all localize to distinct ER domains. A prominent association of LiDGAT1 with ER domains in close proximity to forming lipid droplets (LDs) was also observed. Conclusions The data revealed a distinct molecular, functional and cellular nature of type 1 and type 2 DGATs from L. incisa, with LiDGAT1 being a major contributor to the TAG pool. LiDGATs of type 2 might be in turn involved in the incorporation of unusual fatty acids into TAG and thus regulate the composition of TAG. This report provides a valuable resource for the further research of microalgae DGATs oriented towards production of fresh-water strains with higher oil content of valuable composition, not only for oil industry but also for human and animal nutrition. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1471-2229 |
| Relation: |
http://link.springer.com/article/10.1186/s12870-018-1510-3; https://doaj.org/toc/1471-2229 |
| DOI: |
10.1186/s12870-018-1510-3 |
| URL الوصول: |
https://doaj.org/article/ae765d6a38dc44bfa23146b2587839bd |
| رقم الانضمام: |
edsdoj.765d6a38dc44bfa23146b2587839bd |
| قاعدة البيانات: |
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