التفاصيل البيبلوغرافية
| العنوان: |
Preparation of ICA-loaded mPEG-ICA nanoparticles and their application in the treatment of LPS-induced H9c2 cell damage |
| المؤلفون: |
Lin Zhou, Zhi Huang, Shanyi Yang, Jiarui Wei, Yan Xu, Lin Hu, Xinrong Guo, Limin Yuan, Zexuan Yuan, Xiaoping Yang, Xiaojun Tao, Qiufang Zhang |
| المصدر: |
Nanoscale Research Letters, Vol 16, Iss 1, Pp 1-16 (2021) |
| بيانات النشر: |
SpringerOpen, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Materials of engineering and construction. Mechanics of materials |
| مصطلحات موضوعية: |
Icariin (ICA), Polyethylene glycol monomethyl ether (mPEG), Fourier infrared spectroscopy, Nuclear magnetic resonance spectroscopy (NMR), Dynamic light scattering (DLS), Materials of engineering and construction. Mechanics of materials, TA401-492 |
| الوصف: |
Abstract Hydrophilic polyethylene glycol monomethyl ether (mPEG) was grafted onto Icariin (ICA) by succinic anhydride to form a polyethylene glycol-Icariin (mPEG-ICA) polymer. The structure of the polymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). mPEG-ICA nanoparticles loaded with ICA were prepared by physical embedding of ICA by dialysis. The particle size was determined to be (220 ± 13.7) nm, and the ζ potential was (2.30 ± 1.33) mV by dynamic light scattering (DLS). Under a transmission electron microscope (TEM), the nanoparticles were spherical, and the morphology was regular. In the medium with pH 7.4, the drug release rate of mPEG-ICA nanoparticles reached (52.80 ± 1.70)% within 72 h. At pH 6.8, the cumulative drug release of nanoparticles reached (75.66 ± 0.17)% within 48 h. Treatment of the nanoparticles with LPS-treated H9c2 cells maintained cell viability, reduced LDH release and exerted antiapoptotic effects. Moreover, ICA-loaded mPEG-ICA nanoparticles significantly decreased the mRNA expression of the myocardial inflammatory cytokines TNF-α, IL-1β and IL-6M. In conclusion, ICA-loaded mPEG-ICA nanoparticles protected against LPS-induced H9c2 cell injury. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1556-276X |
| Relation: |
https://doaj.org/toc/1556-276X |
| DOI: |
10.1186/s11671-021-03609-9 |
| URL الوصول: |
https://doaj.org/article/75ff7c3be9c4421581cf45224697be04 |
| رقم الانضمام: |
edsdoj.75ff7c3be9c4421581cf45224697be04 |
| قاعدة البيانات: |
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