Academic Journal
Development of a Two Triplex Real-Time Polymerase Chain Reaction for Rapid Detection of Six Carbapenemase Genes in Enterobacteriaceae
| العنوان: | Development of a Two Triplex Real-Time Polymerase Chain Reaction for Rapid Detection of Six Carbapenemase Genes in Enterobacteriaceae |
|---|---|
| المؤلفون: | Ji Ae Choi, Song Mee Bae, Jung Wook Kim, Kwang Jun Lee |
| المصدر: | Osong Public Health and Research Perspectives, Vol 11, Iss 1, Pp 53-59 (2020) |
| بيانات النشر: | Korea Disease Control and Prevention Agency, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Special situations and conditions LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | carbapenemase, real-time pcr, triplex pcr, Special situations and conditions, RC952-1245, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Objectives Carbapenem resistance is a serious clinical and public health threat. Carbapenemase can confer carbapenem resistance, and most carbapenemase genes are plasmid encoded so resistance can easily spread. In this study, we aimed to develop a novel system based on the TaqMan platform for the rapid detection of 6 clinically prevalent carbapenemase genes: Klebsiella pneumoniae carbapenemase, New Delhi metallo-β-lactamase, oxacillinase, imipenem-hydrolyzing, Verona integron-encoded metallo-β-lactamase, and Guiana extended-spectrum β-lactamase. Methods The triplex assay was verified by testing genomic DNA of 6 carbapenemase-producing Klebsiella pneumoniae. It was validated with a blinded panel of 310 Enterobacteriaceae isolates, including 225 carbapenemase-producers and 85 non-producers, by direct colony triplex real-time polymerase chain reaction (PCR). The real-time PCR was performed using the ABI 7500 fast instrument (Applied Biosystems, CA, USA) and specific primers for each carbapenemase target were designed to include modified peptide-nucleic acid oligonucleotides. Results No amplification was detected among the negative samples. The result showed 100% concordance with the genotypes previously identified. The entire assay, including DNA extraction and real-time PCR, was completed within 2 hours. Conclusion The newly developed triplex real-time PCR assay was useful for the rapid, accurate and simultaneous detection of 6 carbapenemase genes in Enterobacteriaceae, suggesting its potential to allow an early decision on the appropriate treatment, management, and prevention of the spread of resistant infections in hospitals. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2210-9099 |
| Relation: | http://ophrp.org/upload/pdf/ophrp-11-53.pdf; https://doaj.org/toc/2210-9099 |
| DOI: | 10.24171/j.phrp.2020.11.1.08 |
| URL الوصول: | https://doaj.org/article/75b5012f95eb4fd38cba9d84a9731d71 |
| رقم الانضمام: | edsdoj.75b5012f95eb4fd38cba9d84a9731d71 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22109099 |
|---|---|
| DOI: | 10.24171/j.phrp.2020.11.1.08 |