التفاصيل البيبلوغرافية
| العنوان: |
Phenol-Soluble Modulin α3 Stimulates Autophagy in HaCaT Keratinocytes |
| المؤلفون: |
Áron Dernovics, György Seprényi, Zsolt Rázga, Ferhan Ayaydin, Zoltán Veréb, Klára Megyeri |
| المصدر: |
Biomedicines, Vol 11, Iss 11, p 3018 (2023) |
| بيانات النشر: |
MDPI AG, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
pore-forming toxin, staphylococci, phenol-soluble modulin, autophagy, keratinocyte, Biology (General), QH301-705.5 |
| الوصف: |
Background: Phenol-soluble modulins (PSMs) are pore-forming toxins (PFTs) produced by staphylococci. PSMs exert diverse cellular effects, including lytic, pro-apoptotic, pro-inflammatory and antimicrobial actions. Since the effects of PSMs on autophagy have not yet been reported, we evaluated the autophagic activity in HaCaT keratinocytes treated with recombinant PSMα3. Methods: The autophagic flux and levels of autophagic marker proteins were determined using Western blot analysis. Subcellular localization of LC3B and Beclin-1 was investigated using an indirect immunofluorescence assay. The ultrastructural features of control and PSMα3-treated cells were evaluated via transmission electron microscopy. Cytoplasmic acidification was measured via acridine orange staining. Phosphorylation levels of protein kinases, implicated in autophagy regulation, were studied using a phospho-kinase array and Western blot analysis. Results: PSMα3 facilitated the intracellular redistribution of LC3B, increased the average number of autophagosomes per cell, promoted the development of acidic vesicular organelles, elevated the levels of LC3B-II, stimulated autophagic flux and triggered a significant decrease in the net autophagic turnover rate. PSMα3 induced the accumulation of autophagosomes/autolysosomes, amphisomes and multilamellar bodies at the 0.5, 6 and 24 h time points, respectively. The phospho-Akt1/2/3 (T308 and S473), and phospho-mTOR (S2448) levels were decreased, whereas the phospho-Erk1/2 (T202/Y204 and T185/Y187) level was increased in PSMα3-treated cells. Conclusions: In HaCaT keratinocytes, PSMα3 stimulates autophagy. The increased autophagic activity elicited by sub-lytic PSM concentrations might be an integral part of the cellular defense mechanisms protecting skin homeostasis. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2227-9059 |
| Relation: |
https://www.mdpi.com/2227-9059/11/11/3018; https://doaj.org/toc/2227-9059 |
| DOI: |
10.3390/biomedicines11113018 |
| URL الوصول: |
https://doaj.org/article/759fcb9385484133900e1ccca9a581bc |
| رقم الانضمام: |
edsdoj.759fcb9385484133900e1ccca9a581bc |
| قاعدة البيانات: |
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