Academic Journal
Neuronal Cell-type Engineering by Transcriptional Activation
| العنوان: | Neuronal Cell-type Engineering by Transcriptional Activation |
|---|---|
| المؤلفون: | Songlei Liu, Johannes Striebel, Giovanni Pasquini, Alex H. M. Ng, Parastoo Khoshakhlagh, George M. Church, Volker Busskamp |
| المصدر: | Frontiers in Genome Editing, Vol 3 (2021) |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | ORF, neuron, forward programming, transcription factor, CRISPRa, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Gene activation with the CRISPR-Cas system has great implications in studying gene function, controlling cellular behavior, and modulating disease progression. In this review, we survey recent studies on targeted gene activation and multiplexed screening for inducing neuronal differentiation using CRISPR-Cas transcriptional activation (CRISPRa) and open reading frame (ORF) expression. Critical technical parameters of CRISPRa and ORF-based strategies for neuronal programming are presented and discussed. In addition, recent progress on in vivo applications of CRISPRa to the nervous system are highlighted. Overall, CRISPRa represents a valuable addition to the experimental toolbox for neuronal cell-type programming. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2673-3439 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fgeed.2021.715697/full; https://doaj.org/toc/2673-3439 |
| DOI: | 10.3389/fgeed.2021.715697 |
| URL الوصول: | https://doaj.org/article/75507b52e26a4dcd9d24a861e8555c2a |
| رقم الانضمام: | edsdoj.75507b52e26a4dcd9d24a861e8555c2a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26733439 |
|---|---|
| DOI: | 10.3389/fgeed.2021.715697 |