Academic Journal
Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft
| العنوان: | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
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| المؤلفون: | Elza Ibrahim Auerkari, Yolanda Bilynov, Mindya Yuniastuti, Listyowati Listyowati, Lilies Dwi Sulistyani |
| المصدر: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Vol 21 (2021) |
| بيانات النشر: | Association of Support to Oral Health Research (APESB), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Dentistry |
| مصطلحات موضوعية: | Cleft Palate, Cleft Lip, Genetic Variation, Polymorphism, Genetic, Dentistry, RK1-715 |
| الوصف: | Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1519-0501 1983-4632 |
| Relation: | https://revista.uepb.edu.br/PBOCI/article/view/668; https://doaj.org/toc/1519-0501; https://doaj.org/toc/1983-4632 |
| URL الوصول: | https://doaj.org/article/751715e160db4c5abe2c02b9c3a67fe1 |
| رقم الانضمام: | edsdoj.751715e160db4c5abe2c02b9c3a67fe1 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 15190501 19834632 |
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