التفاصيل البيبلوغرافية
| العنوان: |
Fixation-induced cell blebbing on spread cells inversely correlates with phosphatidylinositol 4,5-bisphosphate level in the plasma membrane |
| المؤلفون: |
Siyuan Zhao, Huanhuan Liao, Meiying Ao, Li Wu, Xiaojun Zhang, Yong Chen |
| المصدر: |
FEBS Open Bio, Vol 4, Iss C, Pp 190-199 (2014) |
| بيانات النشر: |
Wiley, 2014. |
| سنة النشر: |
2014 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Cell fixation, Cell blebbing, Phosphatidylinositol 4,5-bisphosphate (PIP2), Human umbilical vein endothelial cells (HUVECs), Lipid rafts, THP-1-derived macrophages, Biology (General), QH301-705.5 |
| الوصف: |
While most attention has been focused on physiologically generated blebs, the molecular mechanisms for fixation-induced cell blebbing are less investigated. We show that protein-fixing (e.g. aldehydes and picric acid) but not lipid-stabilizing (e.g. OsO4 and KMnO4) fixatives induce blebbing on spread cells. We also show that aldehyde fixation may induce the loss or delocalization of phosphatidylinositol 4,5-bisphosphate (PIP2) in the plasma membrane and that the asymmetric distribution of fixation-induced blebs on spread/migrating cells coincides with that of PIP2 on the cells prefixed by lipid-stabilizing fixatives (e.g., OsO4). Moreover, fixation induces blebbing less readily on PIP2-elevated spread cells but more readily on PIP2-lowered or lipid raft-disrupted spread cells. Our data suggest that fixation-induced lowering of PIP2 level at cytoskeleton-attaching membrane sites causes bleb formation via local breakdown of the membrane–cytoskeleton coupling. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2211-5463 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S221154631400014X; https://doaj.org/toc/2211-5463 |
| DOI: |
10.1016/j.fob.2014.02.003 |
| URL الوصول: |
https://doaj.org/article/73fe43e271ab4f469d45096f37405ab7 |
| رقم الانضمام: |
edsdoj.73fe43e271ab4f469d45096f37405ab7 |
| قاعدة البيانات: |
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