Academic Journal
Utility of Conventional ZN Staining and Modified Bleach Techniques in Detection of Acid Fast Bacilli from Aspirates of Suspected Cases of Tuberculous Lymphadenitis
| العنوان: | Utility of Conventional ZN Staining and Modified Bleach Techniques in Detection of Acid Fast Bacilli from Aspirates of Suspected Cases of Tuberculous Lymphadenitis |
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| المؤلفون: | Anjali Shandilya, Indrani Krishnappa, A Mohamed Hamza |
| المصدر: | National Journal of Laboratory Medicine, Vol 13, Iss 2, Pp PO21-PO26 (2024) |
| بيانات النشر: | JCDR Research and Publications Pvt. Ltd., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology LCC:Chemistry |
| مصطلحات موضوعية: | cartridge-based nucleic acid amplification test, conventional ziehl-neelsen staining, fine needle aspiration, modified bleach concentration method, sodium hypochlorite, tuberculosis, Microbiology, QR1-502, Chemistry, QD1-999 |
| الوصف: | Introduction: Tuberculosis (TB) is a significant public health problem in many developing countries, including India. Diagnosing tuberculous lymphadenitis, in particular, poses challenges as it requires the demonstration of Acid-Fast Bacilli (AFB) using the conventional Ziehl-Neelsen (ZN) technique. The Modified Bleach Technique, which involves liquefying the aspirated specimen with sodium hypochlorite followed by centrifugation, has been described to enhance the yield of AFB, improving sensitivity and diagnostic accuracy. Aim: To highlight the role of the bleach concentration method compared to conventional ZN staining in detecting AFB in Fine-Needle Aspiration (FNA) material from clinically suspected cases of tuberculous lymphadenitis. Materials and Methods: A prospective cross-sectional study was conducted at the Department of Pathology, MVJ Medical College and Research Hospital, Bengaluru, Karnataka, India from November 2020 to December 2022. Fine needle aspiration material from 100 cases of clinically suspected tuberculous lymphadenitis was included. In each case, part of the aspirate was processed for ZN staining, while the remaining material was used for modified bleach staining and Cartridge Based Nucleic Acid Amplification Test (CBNAAT). Patient data, including age, sex, duration and site of swelling, nature of aspirate, and cytomorphological diagnosis, were documented. The data was entered into a Microsoft excel spreadsheet and analysed using the Chi-square test, with a p-value |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2277-8551 2455-6882 |
| Relation: | https://www.njlm.net/articles/PDF/2833/64664_CE[Ra1]_F(KR)_QC(AN_RDW_SHU)_PF1(RI_SHU)_PFA_NC(SHU)_PN(SHU).pdf; https://doaj.org/toc/2277-8551; https://doaj.org/toc/2455-6882 |
| DOI: | 10.7860/NJLM/2024/64664.2833 |
| URL الوصول: | https://doaj.org/article/7366c227abba4c0cb8df0a5c24bb74df |
| رقم الانضمام: | edsdoj.7366c227abba4c0cb8df0a5c24bb74df |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 22778551 24556882 |
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| DOI: | 10.7860/NJLM/2024/64664.2833 |