Academic Journal
Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells
| العنوان: | Naturally circulating pertactin-deficient Bordetella pertussis strains induce distinct gene expression and inflammatory signatures in human dendritic cells |
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| المؤلفون: | Michiel M. Kroes, Alberto Miranda-Bedate, Elise S. Hovingh, Ronald Jacobi, Corrie Schot, Elder Pupo, René H. M. Raeven, Arno A. J. van der Ark, Jos P. M. van Putten, Jelle de Wit, Rob Mariman, Elena Pinelli |
| المصدر: | Emerging Microbes and Infections, Vol 10, Iss 1, Pp 1358-1368 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Pathogen adaptation, pertactin, dendritic cell, transcriptomics, RNAseq, proteomics, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | Respiratory infections caused by Bordetella pertussis are reemerging despite high pertussis vaccination coverage. Since the introduction of the acellular pertussis vaccine in the late twentieth century, circulating B. pertussis strains increasingly lack expression of the vaccine component pertactin (Prn). In some countries, up to 90% of the circulating B. pertussis strains are deficient in Prn. To better understand the resurgence of pertussis, we investigated the response of human monocyte-derived dendritic cells (moDCs) to naturally circulating Prn-expressing (Prn-Pos) and Prn-deficient (Prn-Neg) B. pertussis strains from 2016 in the Netherlands. Transcriptome analysis of moDC showed enriched IFNα response-associated gene expression after exposure to Prn-Pos B. pertussis strains, whereas the Prn-Neg strains induced enriched expression of interleukin- and TNF-signaling genes, as well as other genes involved in immune activation. Multiplex immune assays confirmed enhanced proinflammatory cytokine secretion by Prn-Neg stimulated moDC. Comparison of the proteomes from the Prn-Pos and Prn-Neg strains revealed, next to the difference in Prn, differential expression of a number of other proteins including several proteins involved in metabolic processes. Our findings indicate that Prn-deficient B. pertussis strains induce a distinct and stronger immune activation of moDCs than the Prn-Pos strains. These findings highlight the role of pathogen adaptation in the resurgence of pertussis as well as the effects that vaccine pressure can have on a bacterial population. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2021.1943537 |
| URL الوصول: | https://doaj.org/article/7319d35168614a18a604c01201d248a7 |
| رقم الانضمام: | edsdoj.7319d35168614a18a604c01201d248a7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2021.1943537 |