Academic Journal
Defective lytic transglycosylase disrupts cell morphogenesis by hindering cell wall de-O-acetylation in Neisseria meningitidis
| العنوان: | Defective lytic transglycosylase disrupts cell morphogenesis by hindering cell wall de-O-acetylation in Neisseria meningitidis |
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| المؤلفون: | Allison Hillary Williams, Richard Wheeler, Ala-Eddine Deghmane, Ignacio Santecchia, Ryan E Schaub, Samia Hicham, Maryse Moya Nilges, Christian Malosse, Julia Chamot-Rooke, Ahmed Haouz, Joseph P Dillard, William P Robins, Muhamed-Kheir Taha, Ivo Gomperts Boneca |
| المصدر: | eLife, Vol 9 (2020) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Neisseria meningitidis, peptidoglycan, Lytic transglycosylase, cell division, cell separation, X-ray crystallography, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Lytic transglycosylases (LT) are enzymes involved in peptidoglycan (PG) remodeling. However, their contribution to cell-wall-modifying complexes and their potential as antimicrobial drug targets remains unclear. Here, we determined a high-resolution structure of the LT, an outer membrane lipoprotein from Neisseria species with a disordered active site helix (alpha helix 30). We show that deletion of the conserved alpha-helix 30 interferes with the integrity of the cell wall, disrupts cell division, cell separation, and impairs the fitness of the human pathogen Neisseria meningitidis during infection. Additionally, deletion of alpha-helix 30 results in hyperacetylated PG, suggesting this LtgA variant affects the function of the PG de-O-acetylase (Ape 1). Our study revealed that Ape 1 requires LtgA for optimal function, demonstrating that LTs can modulate the activity of their protein-binding partner. We show that targeting specific domains in LTs can be lethal, which opens the possibility that LTs are useful drug-targets. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/51247; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.51247 |
| URL الوصول: | https://doaj.org/article/72f48671227f473d8dc36f8870e383a4 |
| رقم الانضمام: | edsdoj.72f48671227f473d8dc36f8870e383a4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.51247 |