التفاصيل البيبلوغرافية
| العنوان: |
Podoplanin Drives Motility of Active Macrophage via Regulating Filamin C During Helicobacter pylori Infection |
| المؤلفون: |
Yi Ying Cheok, Grace Min Yi Tan, Keith Conrad Fernandez, Yee Teng Chan, Chalystha Yie Qin Lee, Heng Choon Cheong, Chung Yeng Looi, Jamuna Vadivelu, Suhailah Abdullah, Won Fen Wong |
| المصدر: |
Frontiers in Immunology, Vol 12 (2021) |
| بيانات النشر: |
Frontiers Media S.A., 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Immunologic diseases. Allergy |
| مصطلحات موضوعية: |
podoplanin, macrophage, Helicobacter pylori, cell migration, Filamin C, interleukin-1β, Immunologic diseases. Allergy, RC581-607 |
| الوصف: |
Podoplanin (Pdpn) is a mucin-type transmembrane protein that has been implicated in multiple physiological settings including lymphangiogenesis, platelet aggregation, and cancer metastasis. Here, we reported an absence of Pdpn transcript expression in the resting mouse monocytic macrophages, RAW264.7 cells; intriguingly, a substantial upregulation of Pdpn was observed in activated macrophages following Helicobacter pylori or lipopolysaccharide stimulation. Pdpn-knockout macrophages demonstrated intact phagocytic and intracellular bactericidal activities comparable to wild type but exhibited impaired migration due to attenuated filopodia formation. In contrast, an ectopic expression of Pdpn augmented filopodia protrusion in activated macrophages. NanoString analysis uncovered a close dependency of Filamin C gene on the presence of Pdpn, highlighting an involvement of Filamin C in modulation of actin polymerization activity, which controls cell filopodia formation and migration. In addition, interleukin-1β production was significantly declined in the absence of Pdpn, suggesting a role of Pdpn in orchestrating inflammation during H. pylori infection besides cellular migration. Together, our findings unravel the Pdpn network that modulates movement of active macrophages. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-3224 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.702156/full; https://doaj.org/toc/1664-3224 |
| DOI: |
10.3389/fimmu.2021.702156 |
| URL الوصول: |
https://doaj.org/article/e71210880dc34021824110fac3519365 |
| رقم الانضمام: |
edsdoj.71210880dc34021824110fac3519365 |
| قاعدة البيانات: |
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