Academic Journal
Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil
| العنوان: | Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil |
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| المؤلفون: | Keyla Carstens Marques de Sousa, Marcos Rogério André, Heitor Miraglia Herrera, Gisele Braziliano de Andrade, Marcia Mariza Gomes Jusi, Luciana Ladislau dos Santos, Wanessa Teixeira Gomes Barreto, Rosangela Zacarias Machado, Gilson Pereira de Oliveira |
| المصدر: | Revista Brasileira de Parasitologia Veterinária, Vol 22, Iss 4, Pp 525-531 |
| بيانات النشر: | Colégio Brasileiro de Parasitologia Veterinaria. |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | Ehrlichia canis, Babesia vogeli, Anaplasma platys, Leishmania infantum, dogs, co-infection, Animal culture, SF1-1100 |
| الوصف: | Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detectEhrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis ofL. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies toEhrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, forE. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys andLeishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1984-2961 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000400525&lng=en&tlng=en; https://doaj.org/toc/1984-2961 |
| DOI: | 10.1590/S1984-29612013000400012 |
| URL الوصول: | https://doaj.org/article/710118bc6e2f452d88ff9d0c18287a6b |
| رقم الانضمام: | edsdoj.710118bc6e2f452d88ff9d0c18287a6b |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 19842961 |
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| DOI: | 10.1590/S1984-29612013000400012 |