Academic Journal
Scarabaeid Larvae- and Herbicide-Resistant Transgenic Perennial Ryegrass (Lolium perenne L.) Obtained by Agrobacterium tumefaciens-Mediated Transformation of cry8Ca2, cry8Ga and bar Genes
| العنوان: | Scarabaeid Larvae- and Herbicide-Resistant Transgenic Perennial Ryegrass (Lolium perenne L.) Obtained by Agrobacterium tumefaciens-Mediated Transformation of cry8Ca2, cry8Ga and bar Genes |
|---|---|
| المؤلفون: | Jin-xia WU, Zhi-guo ZHANG, Qian ZHANG, Zhi-hong LANG, Xue-hui SUN |
| المصدر: | Journal of Integrative Agriculture, Vol 11, Iss 1, Pp 53-61 (2012) |
| بيانات النشر: | Elsevier, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Agriculture (General) |
| مصطلحات موضوعية: | Agrobacterium tumefaciens-mediated transformation, transgenic perennial ryegrass, scarab larvae resistance, herbicide resistance, Agriculture (General), S1-972 |
| الوصف: | Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicideresistant transgenic perennial ryegrass (Lolium perenne L.) was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene (cry8Ca2 or cry8Ga) and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta?, while leaves of the wild plants wilted after Basta? spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2095-3119 1671-2927 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1671292712607822; https://doaj.org/toc/2095-3119 |
| DOI: | 10.1016/S1671-2927(12)60782-2 |
| URL الوصول: | https://doaj.org/article/70b9b67ba5914beab73ddc243d683d03 |
| رقم الانضمام: | edsdoj.70b9b67ba5914beab73ddc243d683d03 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20953119 16712927 |
|---|---|
| DOI: | 10.1016/S1671-2927(12)60782-2 |