Academic Journal
High-resolution melting analysis to discriminate between the SARS-CoV-2 Omicron variants BA.1 and BA.2
| العنوان: | High-resolution melting analysis to discriminate between the SARS-CoV-2 Omicron variants BA.1 and BA.2 |
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| المؤلفون: | Takuro Koshikawa, Hiroshi Miyoshi |
| المصدر: | Biochemistry and Biophysics Reports, Vol 31, Iss , Pp 101306- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | SARS-CoV-2, Omicron, RBD, HRM analysis, Subvariant, Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | High-resolution melting (HRM) analysis was conducted to discriminate between SARS-CoV-2 Omicron variant BA.1 (B.1.1.529.1) and subvariant BA.2 (B.1.1.529.2). We performed two-step PCR consisting of the first PCR and the second nested PCR to prepare the amplicon for HRM analysis, which detected G339D, N440K, G446S and D796Y variations in the SARS-CoV-2 spike protein. The melting temperatures (Tms) of the amplicons from the cDNA of the Omicron variant BA.1 and subvariant BA.2 receptor binding domain (RBD) in spike protein were the same: 75.2 °C (G339D variation) and 73.4 °C (D796Y variation). These Tms were distinct from those of SARS-CoV-2 isolate Wuhan-Hu-1, and were specific to the Omicron variant. In HRM analyses that detected the N440K and G446S variations, the Tms of amplicons from the cDNA of the Omicron variant BA.1 and subvariant BA.2 RBDs were 73.0 °C (N440K and G446S variations) and 73.5 °C (G446S variation). This difference indicates that the SARS-CoV-2 Omicron variants BA.1 and BA.2 can be clearly discriminated. Our study demonstrates the usefulness of HRM analysis after two-step PCR for the discrimination of SARS-CoV-2 variants. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580822001066; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2022.101306 |
| URL الوصول: | https://doaj.org/article/70224dab100f4cbf9c927baaca3c3b23 |
| رقم الانضمام: | edsdoj.70224dab100f4cbf9c927baaca3c3b23 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2022.101306 |