Academic Journal
Identifying molecular features that are associated with biological function of intrinsically disordered protein regions
| العنوان: | Identifying molecular features that are associated with biological function of intrinsically disordered protein regions |
|---|---|
| المؤلفون: | Taraneh Zarin, Bob Strome, Gang Peng, Iva Pritišanac, Julie D Forman-Kay, Alan M Moses |
| المصدر: | eLife, Vol 10 (2021) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | IDRs, lasso, IRLS, evolutionary signatures, logistic regression, Expectation-Maximization, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | In previous work, we showed that intrinsically disordered regions (IDRs) of proteins contain sequence-distributed molecular features that are conserved over evolution, despite little sequence similarity that can be detected in alignments (Zarin et al., 2019). Here, we aim to use these molecular features to predict specific biological functions for individual IDRs and identify the molecular features within them that are associated with these functions. We find that the predictable functions are diverse. Examining the associated molecular features, we note some that are consistent with previous reports and identify others that were previously unknown. We experimentally confirm that elevated isoelectric point and hydrophobicity, features that are positively associated with mitochondrial localization, are necessary for mitochondrial targeting function. Remarkably, increasing isoelectric point in a synthetic IDR restores weak mitochondrial targeting. We believe feature analysis represents a new systematic approach to understand how biological functions of IDRs are specified by their protein sequences. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/60220; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.60220 |
| URL الوصول: | https://doaj.org/article/a6e4dce7bd0e4410a47cefd1282bcbdc |
| رقم الانضمام: | edsdoj.6e4dce7bd0e4410a47cefd1282bcbdc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
|---|---|
| DOI: | 10.7554/eLife.60220 |