Academic Journal
Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins.
| العنوان: | Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins. |
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| المؤلفون: | Yuishin Kosaka, Wataru Aoki, Megumi Mori, Shunsuke Aburaya, Yuta Ohtani, Hiroyoshi Minakuchi, Mitsuyoshi Ueda |
| المصدر: | PLoS ONE, Vol 15, Iss 12, p e0236850 (2020) |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Ribosomes are the sophisticated machinery that is responsible for protein synthesis in a cell. Recently, quantitative mass spectrometry (qMS) have been successfully applied for understanding the dynamics of protein complexes. Here, we developed a highly specific and reproducible method to quantify all ribosomal proteins (r-proteins) by combining selected reaction monitoring (SRM) and isotope labeling. We optimized the SRM methods using purified ribosomes and Escherichia coli lysates and verified this approach as detecting 41 of the 54 r-proteins separately synthesized in E. coli S30 extracts. The SRM methods will enable us to utilize qMS as a highly specific analytical tool in the research of E. coli ribosomes, and this methodology have potential to accelerate the understanding of ribosome biogenesis, function, and the development of engineered ribosomes with additional functions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0236850 |
| URL الوصول: | https://doaj.org/article/d6db74887d9441768afc05fe0debd465 |
| رقم الانضمام: | edsdoj.6db74887d9441768afc05fe0debd465 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0236850 |