Academic Journal
Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain
| العنوان: | Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain |
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| المؤلفون: | Yan Xin, Hu Wei, Zhu Yun-Guo, Liu Qing-Qing, Wang Shuai, Liu Hong-Yan, Zhu Dan, Lv Zhi-Hua, Li Lin-Hua, Yin Yi-Rui |
| المصدر: | Polish Journal of Microbiology, Vol 72, Iss 3, Pp 277-283 (2023) |
| بيانات النشر: | Sciendo, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Genetics LCC:Microbiology |
| مصطلحات موضوعية: | cangshan mountain, uricase-producing strain, arthrobacter sp., heterologous expression, neutral uricase, Genetics, QH426-470, Microbiology, QR1-502 |
| الوصف: | Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as Arthrobacter sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named aruox) of Arthrobacter sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa. The optimal pH and temperature of ArUOX were pH 7 and 20°C, respectively. The ArUOX remained above 50% relative activity after incubation at 37°C for 100 min or at pH 6.0–8.6 for 24 h. Moreover, metal ions such as K+, Mg2+, Ca2+, Ba2+ and Pb2+ can significantly enhance the activity of ArUOX (> 200%). These enzymatic properties indicate that ArUOX has potential applications in pharmaceutical enzymes and uric acid detection kits. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2544-4646 |
| Relation: | https://doaj.org/toc/2544-4646 |
| DOI: | 10.33073/pjm-2023-027 |
| URL الوصول: | https://doaj.org/article/6da47f4159e144878ec550ed052f3729 |
| رقم الانضمام: | edsdoj.6da47f4159e144878ec550ed052f3729 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25444646 |
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| DOI: | 10.33073/pjm-2023-027 |