Academic Journal
Enhanced non-viral gene delivery via calcium phosphate/DNA co-precipitates with low-voltage pulse electroporation in NK-92 cells for immunocellular therapy
| العنوان: | Enhanced non-viral gene delivery via calcium phosphate/DNA co-precipitates with low-voltage pulse electroporation in NK-92 cells for immunocellular therapy |
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| المؤلفون: | Che-Yung Kuan, I-Hsuan Yang, Chia-Ting Chang, Zhi-Yu Chen, Jhih-Ni Lin, Wei-Ting Kuo, Yu-Ying Lin, Andrew Yueh, Feng-Huei Lin |
| المصدر: | APL Bioengineering, Vol 8, Iss 3, Pp 036107-036107-10 (2024) |
| بيانات النشر: | AIP Publishing LLC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Achieving high cell transfection efficiency is essential for various cell types in numerous disease applications. However, the efficient introduction of genes into natural killer (NK) cells remains a challenge. In this study, we proposed a design strategy for delivering exogenous genes into the NK cell line, NK-92, using a modified non-viral gene transfection method. Calcium phosphate/DNA nanoparticles (pDNA-CaP NPs) were prepared using co-precipitation methods and combined with low-voltage pulse electroporation to facilitate NK-92 transfection. The results demonstrated that the developed pDNA-CaP NPs exhibited a uniform diameter of approximately 393.9 nm, a DNA entrapment efficiency of 65.8%, and a loading capacity of 15.9%. Furthermore, at three days post-transfection, both the transfection efficiency and cell viability of NK-92 were significantly improved compared to standalone plasmid DNA (pDNA) electroporation or solely relying on the endocytosis pathway of pDNA-CaP NPs. This study provides valuable insights into a novel approach that combines calcium phosphate nanoparticles with low-voltage electroporation for gene delivery into NK-92 cells, offering potential advancements in cell therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2473-2877 |
| Relation: | https://doaj.org/toc/2473-2877 |
| DOI: | 10.1063/5.0198191 |
| URL الوصول: | https://doaj.org/article/6b481033e6a04da3aeb792a432dbd836 |
| رقم الانضمام: | edsdoj.6b481033e6a04da3aeb792a432dbd836 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24732877 |
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| DOI: | 10.1063/5.0198191 |