التفاصيل البيبلوغرافية
| العنوان: |
A double-negative feedback loop between NtrBC and a small RNA rewires nitrogen metabolism in legume symbionts |
| المؤلفون: |
Natalia I. García-Tomsig, Fernando M. García-Rodriguez, Sabina K. Guedes-García, Vicenta Millán, Anke Becker, Marta Robledo, José I. Jiménez-Zurdo |
| المصدر: |
mBio, Vol 14, Iss 6 (2023) |
| بيانات النشر: |
American Society for Microbiology, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
riboregulation, rhizobia, alpha-proteobacteria, nitrogen fixation, Sinorhizobium meliloti, two-component regulatory systems, Microbiology, QR1-502 |
| الوصف: |
ABSTRACTThe nitrogen (N) status transduced via the NtrBC two-component system is a major signaling cue in the root nodule endosymbiosis of diazotrophic rhizobia with legumes. NtrBC is upregulated in the N-limiting rhizosphere environment at the onset of nodulation but silenced in nodules to favor the assimilation of the fixed N into plant biomass. We reported that the trans-acting sRNA NfeR1 (Nodule Formation Efficiency RNA) broadly influences the symbiotic performance of the α-rhizobium Sinorhizobium meliloti. Here, we show that NfeR1 is indeed an N-responsive sRNA that fine-tunes NtrBC output during the symbiotic transition. Biochemical and genetic approaches unveiled that NtrC and the LysR-type symbiotic regulator LsrB bind at distinct nearby sites in the NfeR1 promoter, acting antagonistically as repressor and activator of transcription, respectively. This complex transcriptional control specifies peak NfeR1 steady-state levels in N-starved and endosymbiotic bacteria. Furthermore, NfeR1 base pairs the translation initiation region of the histidine kinase coding mRNA ntrB, causing a decrease in both NtrB and NtrC abundance as assessed by double-plasmid genetic assays. In the context of endogenous regulation, NfeR1-mediated ntrBC silencing most likely amends the effective strength of the known operon autorepression exerted by NtrC. Accordingly, a lack of NfeR1 shifts the wild-type NtrBC output, restraining the fitness of free-living rhizobia under N stress and plant growth upon nodulation. The mixed NtrBC-NfeR1 double-negative feedback loop is thus an unprecedented adaptive network motif that helps α-rhizobia adjust N metabolism to the demands of an efficient symbiosis with legume plants.IMPORTANCERoot nodule endosymbioses between diazotrophic rhizobia and legumes provide the largest input of combined N to the biosphere, thus representing an alternative to harmful chemical fertilizers for sustainable crop production. Rhizobia have evolved intricate strategies to coordinate N assimilation for their own benefit with N2 fixation to sustain plant growth. The rhizobial N status is transduced by the NtrBC two-component system, the seemingly ubiquitous form of N signal transduction in Proteobacteria. Here, we show that the regulatory sRNA NfeR1 (nodule formation efficiency RNA) of the alfalfa symbiont Sinorhizobium meliloti is transcribed from a complex promoter repressed by NtrC in a N-dependent manner and feedback silences ntrBC by complementary base-pairing. These findings unveil a more prominent role of NtrC as a transcriptional repressor than hitherto anticipated and a novel RNA-based mechanism for NtrBC regulation. The NtrBC-NfeR1 double-negative feedback loop accurately rewires symbiotic S. meliloti N metabolism and is likely conserved in α-rhizobia. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2150-7511 |
| Relation: |
https://doaj.org/toc/2150-7511 |
| DOI: |
10.1128/mbio.02003-23 |
| URL الوصول: |
https://doaj.org/article/6aaa4a9c2a44465fa8c223e9a8ea1920 |
| رقم الانضمام: |
edsdoj.6aaa4a9c2a44465fa8c223e9a8ea1920 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder