التفاصيل البيبلوغرافية
| العنوان: |
Increasing the physical size and nucleation status of human pluripotent stem cell-derived ventricular cardiomyocytes by cell fusion |
| المؤلفون: |
Chi-Wing Kong, Shuxun Chen, Lin Geng, Angie Man-Yee Shum, Dong Sun, Ronald A Li |
| المصدر: |
Stem Cell Research, Vol 19, Iss , Pp 76-81 (2017) |
| بيانات النشر: |
Elsevier, 2017. |
| سنة النشر: |
2017 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Human pluripotent stem cell-derived ventricular cardiomyocytes (hPSC-VCMs), Cell fusion, Hypertrophy, Multi-nucleation, Electrophysiological maturation, Biology (General), QH301-705.5 |
| الوصف: |
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) provide an unlimited source of donor cells for potential cardiac regenerative therapies. However, hPSC-CMs are immature. For instance, hPSC-CMs are only 1/10 of the physical size of their adult counterparts; the majority are mono- rather than bi- or multi-nucleated, which is an evolutionary adaptive feature in metabolically active cells such as adult CMs. Here, we attempted to increase the physical size and nucleation status of hPSC-derived ventricular (V) cardiomyocytes (hPSC-VCMs) using chemically-induced cell fusion, and examined the subsequent functional effects. Polyethylene glycol (PEG) was employed to fuse a 1:1 mixture of lentiviral vectors LV-MLC2v-GFP- or –tdTomato-labeled hPSC-VCMs, such that hPSC-VCMs fused syncytia (FS) were identified as doubly GFP+/tdTomato+ multi-nucleated cells. These microscopically-identified FS were doubled in size as gauged by their capacitance when compared to the control mononucleated hPSC-VCMs using patch-clamp analysis. Reduced automaticity or action potential (AP) firing rate and moderately prolonged AP duration were observed in FS from day 6 post-fusion induction. However, Ca2+ handling, mitochondrial biogenesis and the extent of apoptosis were not significantly altered. We conclude that larger, multi-nucleated hPSC-VCMs FS can be created by chemically-induced cell fusion but global maturation requires additional triggering cues. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1873-5061 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S187350611730003X; https://doaj.org/toc/1873-5061 |
| DOI: |
10.1016/j.scr.2017.01.003 |
| URL الوصول: |
https://doaj.org/article/e6a8f84b6cd54bcfb09793d2a8d851cd |
| رقم الانضمام: |
edsdoj.6a8f84b6cd54bcfb09793d2a8d851cd |
| قاعدة البيانات: |
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