Academic Journal
Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery
| العنوان: | Plant lamin-like proteins mediate chromatin tethering at the nuclear periphery |
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| المؤلفون: | Bo Hu, Nan Wang, Xiuli Bi, Ezgi Süheyla Karaaslan, Anna-Lena Weber, Wangsheng Zhu, Kenneth Wayne Berendzen, Chang Liu |
| المصدر: | Genome Biology, Vol 20, Iss 1, Pp 1-18 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Arabidopsis, Lamin, Chromatin structure, Nuclear periphery, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Background The nuclear envelope not only serves as a physical barrier separating nuclear content from the cytoplasm but also plays critical roles in modulating the three-dimensional organization of genomic DNA. For both plants and animals, the nuclear periphery is a functional compartment enriched with heterochromatin. To date, how plants manage to selectively tether chromatin at the nuclear periphery is unclear. Results By conducting dual-color fluorescence in situ hybridization experiments on 2C nuclei, we show that in Arabidopsis thaliana, specific chromatin positioning at the nuclear periphery requires plant lamin-like proteins CROWDED NUCLEI 1 (CRWN1), CRWN4, and DNA methylation in CHG and CHH contexts. With chromosome painting and Hi-C analyses, we show global attenuation of spatial chromatin compartmentalization and chromatin positioning patterns at the nuclear periphery in both the crwn1 and crwn4 mutants. Furthermore, ChIP-seq analysis indicates that CRWN1 directly interacts with chromatin domains localized at the nuclear periphery, which mainly contains non-accessible chromatin. Conclusions In summary, we conclude that CRWN1 is a key component of the lamina-chromatin network in plants. It is functionally equivalent to animal lamins, playing critical roles in modulating patterns of chromatin positioning at the nuclear periphery. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| Relation: | http://link.springer.com/article/10.1186/s13059-019-1694-3; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-019-1694-3 |
| URL الوصول: | https://doaj.org/article/6a6ec428da8c483bace7ed0b2d5b8378 |
| رقم الانضمام: | edsdoj.6a6ec428da8c483bace7ed0b2d5b8378 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-019-1694-3 |