Academic Journal
Human bone marrow mesenchimal stem cells growth rate dynamics, immunophenotype and genetic stability on early and late passages at ex vivo culturing
| العنوان: | Human bone marrow mesenchimal stem cells growth rate dynamics, immunophenotype and genetic stability on early and late passages at ex vivo culturing |
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| المؤلفون: | E. Yu. Osipova, V. A. Nikitina, T. A. Astrelina, A. Yu. Ustyugov, E. V. Dmitrieva, B. B. Purbueva, E. V. Skorobogatova, T. V. Schamanckaya, Z. M. Dischlevaya, M. V. Yakovleva, O. A. Maiorova, L. D. Katosova, S. A. Roumiantsev, N. P. Bochkov |
| المصدر: | Онкогематология, Vol 0, Iss 1, Pp 44-50 (2022) |
| بيانات النشر: | ABV-press, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Diseases of the blood and blood-forming organs |
| مصطلحات موضوعية: | mesenchimal stem cells, passage, immunophenotype, proliferating activity, genetic stability, expansion ex vivo, Diseases of the blood and blood-forming organs, RC633-647.5 |
| الوصف: | Human mesenchimal stem cells (MSC) growth dynamics, immunophenotype change, genetic stability on early (3—4) and late (10—12) passages at in vitro culturing was studied. Results of bone marrow stromal fibroblasts culturing (n=25) from allogeneic transplant recipients with hematological disorders are presented. It has been shown, that in MSC population cultivated in vitro linear homogeneity of cells is observed on 3—4 passage, and remains on 10—12 passage. Clonal heterogeneities of MSC population and selective advantage of certain clones during culturing was revealed as aneuploidy analysis results of cultivated cells. The MSC culturing protocol used in our work allows receiving well characterized MSC number sufficient for clinical application as early as 3—4 passage. While using in the therapeutic purposes MSC later terms of expansion it is necessary to have close control of cells immunophenotype and genetic stability, which will allow avoiding in the future the undesirable long-term consequences of MSC cultures clinical use. The obtained data are discussed and compared with literature data. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 1818-8346 2413-4023 |
| Relation: | https://oncohematology.abvpress.ru/ongm/article/view/710; https://doaj.org/toc/1818-8346; https://doaj.org/toc/2413-4023 |
| DOI: | 10.17650/1818-8346-2009-0-1-44-50 |
| URL الوصول: | https://doaj.org/article/ca68620d7bc149b1b09871045628787f |
| رقم الانضمام: | edsdoj.68620d7bc149b1b09871045628787f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 18188346 24134023 |
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| DOI: | 10.17650/1818-8346-2009-0-1-44-50 |