Academic Journal
Visualization through Magnetic Resonance Imaging of DNA Internalized Following 'In Vivo' Electroporation
| العنوان: | Visualization through Magnetic Resonance Imaging of DNA Internalized Following 'In Vivo' Electroporation |
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| المؤلفون: | Simonetta Geninatti Crich, Stefania Lanzardo, Alessandro Barge, Giovanna Esposito, Lorenzo Tei, Guido Forni, Silvio Aime |
| المصدر: | Molecular Imaging, Vol 4 (2005) |
| بيانات النشر: | SAGE Publishing, 2005. |
| سنة النشر: | 2005 |
| المجموعة: | LCC:Biology (General) LCC:Medical technology |
| مصطلحات موضوعية: | Biology (General), QH301-705.5, Medical technology, R855-855.5 |
| الوصف: | The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, K a = 2.2 × 10 3 M −1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1536-0121 |
| Relation: | https://doaj.org/toc/1536-0121 |
| DOI: | 10.1162/15353500200504151 |
| URL الوصول: | https://doaj.org/article/6811393f5211475f8bca390bf076b66f |
| رقم الانضمام: | edsdoj.6811393f5211475f8bca390bf076b66f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 15360121 |
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| DOI: | 10.1162/15353500200504151 |