Academic Journal
Metabolism of primaquine in normal human volunteers: investigation of phase I and phase II metabolites from plasma and urine using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry
| العنوان: | Metabolism of primaquine in normal human volunteers: investigation of phase I and phase II metabolites from plasma and urine using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry |
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| المؤلفون: | Bharathi Avula, Babu L. Tekwani, Narayan D. Chaurasiya, Pius Fasinu, N. P. Dhammika Nanayakkara, H. M. T. Bhandara Herath, Yan-Hong Wang, Ji-Yeong Bae, Shabana I. Khan, Mahmoud A. Elsohly, James D. McChesney, Peter A. Zimmerman, Ikhlas A. Khan, Larry A. Walker |
| المصدر: | Malaria Journal, Vol 17, Iss 1, Pp 1-14 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Primaquine, Antimalarial, Malaria, Plasmodium vivax, 8-aminoquinoline, Drug metabolism, Arctic medicine. Tropical medicine, RC955-962, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Abstract Background Primaquine (PQ), an 8-aminoquinoline, is the only drug approved by the United States Food and Drug Administration for radical cure and prevention of relapse in Plasmodium vivax infections. Knowledge of the metabolism of PQ is critical for understanding the therapeutic efficacy and hemolytic toxicity of this drug. Recent in vitro studies with primary human hepatocytes have been useful for developing the ultra high-performance liquid chromatography coupled with high-resolution mass spectrometric (UHPLC-QToF-MS) methods for simultaneous determination of PQ and its metabolites generated through phase I and phase II pathways for drug metabolism. Methods These methods were further optimized and applied for phenotyping PQ metabolites from plasma and urine from healthy human volunteers treated with single 45 mg dose of PQ. Identity of the metabolites was predicted by MetaboLynx using LC–MS/MS fragmentation patterns. Selected metabolites were confirmed with appropriate standards. Results Besides PQ and carboxy PQ (cPQ), the major plasma metabolite, thirty-four additional metabolites were identified in human plasma and urine. Based on these metabolites, PQ is viewed as metabolized in humans via three pathways. Pathway 1 involves direct glucuronide/glucose/carbamate/acetate conjugation of PQ. Pathway 2 involves hydroxylation (likely cytochrome P450-mediated) at different positions on the quinoline ring, with mono-, di-, or even tri-hydroxylations possible, and subsequent glucuronide conjugation of the hydroxylated metabolites. Pathway 3 involves the monoamine oxidase catalyzed oxidative deamination of PQ resulting in formation of PQ-aldehyde, PQ alcohol and cPQ, which are further metabolized through additional phase I hydroxylations and/or phase II glucuronide conjugations. Conclusion This approach and these findings augment our understanding and provide comprehensive view of pathways for PQ metabolism in humans. These will advance the clinical studies of PQ metabolism in different populations for different therapeutic regimens and an understanding of the role these play in PQ efficacy and safety outcomes, and their possible relation to metabolizing enzyme polymorphisms. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1475-2875 |
| Relation: | http://link.springer.com/article/10.1186/s12936-018-2433-z; https://doaj.org/toc/1475-2875 |
| DOI: | 10.1186/s12936-018-2433-z |
| URL الوصول: | https://doaj.org/article/654e746afd904edc9c8c9089d7d34a5d |
| رقم الانضمام: | edsdoj.654e746afd904edc9c8c9089d7d34a5d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14752875 |
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| DOI: | 10.1186/s12936-018-2433-z |