Academic Journal
Enhanced visualization of nuclear staining and cell cycle analysis for the human commensal Malassezia
| العنوان: | Enhanced visualization of nuclear staining and cell cycle analysis for the human commensal Malassezia |
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| المؤلفون: | Jayaprakash Sasikumar, Suparna Laha, Bharati Naik, Shankar Prasad Das |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Malassezia, Nuclear staining, Cell cycle, DAPI, Propidium iodide, Nocodazole, Medicine, Science |
| الوصف: | Abstract Malassezia is a lipophilic commensal yeast that resides mainly on the mammalian skin and is also found to associate with the internal organs. Dysbiosis of Malassezia is related to several diseases and often escapes detection as it is difficult to culture and maintain. Malassezia cell wall differs from other budding yeasts like S. cerevisiae due to the difference in the lipid content and is difficult to transform. In this study, we present a methodology to stain Malassezia's nucleus and perform cell cycle studies. However, staining presents a challenge due to its exceptionally thick cell wall with high lipid content, hindering conventional methods. Our novel methodology addresses this challenge and enables the staining of the Malassezia nucleus with a low background. This would allow researchers to visualize the overall nuclear health specifically nuclear morphology and analyze DNA content, crucial for cell cycle progression. By employing DNA-specific dyes like DAPI or Hoechst, we can observe the nuclear structure, and using PI we can differentiate cells in distinct cell cycle phases using techniques like flow cytometry. This novel staining methodology unlocks the door for in-depth cell cycle analysis in Malassezia which has challenged us through ages being refractory to genetic manipulations, paving the way for a deeper understanding of this commensal fungus and its potential role in human health. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-69024-z |
| URL الوصول: | https://doaj.org/article/64bd9b73cc2b46729b50919124115e4a |
| رقم الانضمام: | edsdoj.64bd9b73cc2b46729b50919124115e4a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-69024-z |