التفاصيل البيبلوغرافية
| العنوان: |
Transcriptomic Analysis of Resistant and Susceptible Responses in a New Model Root-Knot Nematode Infection System Using Solanum torvum and Meloidogyne arenaria |
| المؤلفون: |
Kazuki Sato, Taketo Uehara, Julia Holbein, Yuko Sasaki-Sekimoto, Pamela Gan, Takahiro Bino, Katsushi Yamaguchi, Yasunori Ichihashi, Noriko Maki, Shuji Shigenobu, Hiroyuki Ohta, Rochus B. Franke, Shahid Siddique, Florian M. W. Grundler, Takamasa Suzuki, Yasuhiro Kadota, Ken Shirasu |
| المصدر: |
Frontiers in Plant Science, Vol 12 (2021) |
| بيانات النشر: |
Frontiers Media S.A., 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Plant culture |
| مصطلحات موضوعية: |
Turkey berry, plant immunity, plant-parasitic nematode, lignin deposition, comparative transcriptomics, Plant culture, SB1-1110 |
| الوصف: |
Root-knot nematodes (RKNs) are among the most devastating pests in agriculture. Solanum torvum Sw. (Turkey berry) has been used as a rootstock for eggplant (aubergine) cultivation because of its resistance to RKNs, including Meloidogyne incognita and M. arenaria. We previously found that a pathotype of M. arenaria, A2-J, is able to infect and propagate in S. torvum. In vitro infection assays showed that S. torvum induced the accumulation of brown pigments during avirulent pathotype A2-O infection, but not during virulent A2-J infection. This experimental system is advantageous because resistant and susceptible responses can be distinguished within a few days, and because a single plant genome can yield information about both resistant and susceptible responses. Comparative RNA-sequencing analysis of S. torvum inoculated with A2-J and A2-O at early stages of infection was used to parse the specific resistance and susceptible responses. Infection with A2-J did not induce statistically significant changes in gene expression within one day post-inoculation (DPI), but afterward, A2-J specifically induced the expression of chalcone synthase, spermidine synthase, and genes related to cell wall modification and transmembrane transport. Infection with A2-O rapidly induced the expression of genes encoding class III peroxidases, sesquiterpene synthases, and fatty acid desaturases at 1 DPI, followed by genes involved in defense, hormone signaling, and the biosynthesis of lignin at 3 DPI. Both isolates induced the expression of suberin biosynthetic genes, which may be triggered by wounding during nematode infection. Histochemical analysis revealed that A2-O, but not A2-J, induced lignin accumulation at the root tip, suggesting that physical reinforcement of cell walls with lignin is an important defense response against nematodes. The S. torvum-RKN system can provide a molecular basis for understanding plant-nematode interactions. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-462X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fpls.2021.680151/full; https://doaj.org/toc/1664-462X |
| DOI: |
10.3389/fpls.2021.680151 |
| URL الوصول: |
https://doaj.org/article/64775b990b8d45608bfa6e0152833da6 |
| رقم الانضمام: |
edsdoj.64775b990b8d45608bfa6e0152833da6 |
| قاعدة البيانات: |
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