Academic Journal
New Advantageous Method for the Production of Purified Cholera Toxin B-Subunit and Monoclonal Antibodies to It
| العنوان: | New Advantageous Method for the Production of Purified Cholera Toxin B-Subunit and Monoclonal Antibodies to It |
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| المؤلفون: | T. L. Zakharova, E. A. Mikheeva, N. A. Osina |
| المصدر: | Проблемы особо опасных инфекций, Vol 0, Iss 2, Pp 79-82 (2015) |
| بيانات النشر: | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | в-субъединица холерного токсина, гель-хроматография, vibrio cholerae, cholera toxin b-subunit, gel-penetration column chromatography, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Put forward is an efficient method for manufacturing cholera toxin B-subunit. Its advantages are relative simplicity and economy feasibility, as well as maximum output of the purified B-subunit, absolutely free from toxic A-subunit contaminant. All this is due to the deployment of cholera vibrio recombinant strain producing only cholera toxin B-subunit instead of cholera toxin as it is, which results in lack of residual preparation toxicity. Applied has been gel-penetration column chromatography, providing for stable native state and maximum antigen output. The method under discussion is verified experimentally. Sample purity has been analyzed after each phase of chromatographic investigation on TSK gel HW-60, using disc electrophoresis. It is established that three steps of purification are ample for the obtainment of cholera toxin B-subunit preparation free from admixtures. Immunological activity of the purified B-subunit is validated by monoclonal antibody obtainment. Designed preparation of cholera toxin B-subunit and monoclonal antibodies to it can serve as a basis for the development of various immune-diagnostic test-systems alternatives. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Russian |
| تدمد: | 0370-1069 2658-719X |
| Relation: | https://journal.microbe.ru/jour/article/view/232; https://doaj.org/toc/0370-1069; https://doaj.org/toc/2658-719X |
| DOI: | 10.21055/0370-1069-2015-2-79-82 |
| URL الوصول: | https://doaj.org/article/63fbd44a128e4bd796050e674d2004bd |
| رقم الانضمام: | edsdoj.63fbd44a128e4bd796050e674d2004bd |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 03701069 2658719X |
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| DOI: | 10.21055/0370-1069-2015-2-79-82 |